Impact of lifestyle changes on innate immune defense throughout the lifespan

Abstract

Evidence exists that moderate exercise has beneficial effects on immune function throughout the lifespan. Moreover, Withania somnifera (WS), an herb in Ayurvedic medicine, has been investigated for anti-inflammatory properties and stimulatory effect on the immune system for disease resistance. Additionally, an unhealthy diet promotes systemic inflammation. We investigated the hypotheses that voluntary exercise, WS, and a whole-food diet enhances immune function and decreases systemic inflammation throughout the lifespan. The outcome measures were the inflammatory marker C-reactive protein (CRP) and lysozyme. We examined changes due to our interventions outlined above. Male Sprague-Dawley rats were divided into five treatment groups: each interventions, a combined treatment group (all interventions), and control (standard food and no exercise). Analysis of CRP expression was performed using an ELISA and analysis of lysozyme was performed through a lysoplate assay. Differences in expression levels of CRP and lysozyme, amongst different interventions, indicated a possible predictor for monitoring immunosenescence