Recipe for standard BG-11 media v1

Abstract

Stanier RY, Deruelles J, Rippka R, Herdman M, Waterbury JB: Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria. Microbiology 1979, 111:1–61. Recipes for standard and alternative BG11 for culturing freshwater cyanobacteria, such asSynechocystis sp. PCC 6803, as described. Media is usually not suitable for marine cyanobacteria. Final Concentration of Medium. CaCl2*2 H2O 0.036 g/L Citric acid 0.006 g/L NaNO3 1.4958 g/L MgSO4* 7 H2O 0.0749 g/L 0.25M Na2EDTA (pH 8) 0.0056 mL/L Na2CO3 20 µg/L Fe(III) Ammonium citrate 6 µg/L K2HPO4 * 3H2O 30 µg/L TES Buffer (pH 8) 10 mM H3BO3 2.86 mg/L MnCl2 * 4 H2O 1.81 mg/L ZnSO4 * 7 H2O 0.222 mg/L Na2MoO4 * 2 H2O 0.390 mg/L Co(NO3)2 *6 H2O 0.049 mg/L (CuSO4 * 5 H2O 0.079 mg/L if required) </p