Tussilago farfara (L.), an Asteraceae of interest for traditional medicine, is known as a source of valuable essential oil (EO). The chemical composition of hydrodistilled EO of T. farfara flowers and stems was determined by GC and GC/MS techniques. Comparatively, quantitative and qualitative differences were observed in EO composition from both selected origins. The EO yields were 0.07 ± 0.01% and 0.09 ± 0.00% from Lithuanian and French origin. In total, 37 components were identified in Lithuanian and French oils. The major constituent in Lithuanian oil was n-tricosane (21.7 ± 0.1%) while in French oil it was 1-nonene (34.1 ± 0.00%