EFFECT OF METHIONINE, NORLEUCINE, AND LYSINE DERIVATIVES ON CEPHALOSPORIN C FORMATION IN CHEMICALLY DEFINED MEDIA

Abstract

Demain , A. L. (Merck Sharp &amp; Dohme Research Laboratories, Rahway, N.J.), Joanne F. Newkirk, and D. Hendlin . Effect of methionine, norleucine, and lysine derivatives on cephalosporin C formation in chemically defined media. J. Bacteriol. 85: 339–344. 1963.—Chemically defined media were developed for production of cephalosporin C by Cephalosporium sp. In such media, the requirement for methionine can be satisfied by norleucine. Further stimulation of antibiotic production was obtained with the lysine derivatives ε- N -acetyl- l -lysine and ε-aminocaproic acid but not with lysine itself. Also inactive were α-aminoadipic and ketoadipic acids. Other lysine derivatives were found to inhibit cephalosporin C production at 0.01 m . The final medium supported the production of approximately 0.5 g of cephalosporin C per liter of medium. </jats:p