By using various concentrations of auxins and cytokinins it was found that 3 mg/L BAP+lmg/L NAA is ideal for regeneration of the leaflet explants of groundnut.Transformation of the leaflet explants of groundnut with soybean trypsin inhibitor
(SBTI) gene had been done through Agrobacteriwn.The leaflet explants of groundnut had also been transformed with SBTI gene through Biolistics.The putatively transformed leaflet explants were assayed histochemically for the
presence of this gene in the transformants