National Institute of Science Communication and Information Resources
Abstract
A procedure has been described to determine picomole levels
of adenosine3',5'-cyclic monophosphate (CAMP) in plant materials. The purification of CAMP from other
interfering nucleotide and phenolic compounds is made
using paper and column chromatography. The resulting CAMP
is enzymatically transformed to ATP and estimated by its luminescent reaction with iuciferase. The advantages of this method have been discussed in the light of purification and assay of CAMP. The content of CAMP in lettuce, barley seeds, cabbage leaves, and cultivated mushrooms has been estimated by this procedur