Baldwin, R

Foulke, S

Karasev, A V

Koprowski, H

Reitz, M S, Jr

Wellens, C

Zwierzynski, I

Name not available

Orally Delivered, Plant-Produced Tat Protein Primes Mice For a Challenge DNA Vaccine Expressing Tat

Jefferson Digital Commons

Thomas Jefferson UniversityJefferson Digital CommonsDepartment of Microbiology and ImmunologyFaculty Papers Department of Microbiology and Immunology12-8-2005Orally Delivered, Plant-Produced Tat ProteinPrimes Mice For a Challenge DNA VaccineExpressing TatA V. KarasevBiotechnology Foundation Laboratories at Thomas Jefferson University, Department of Microbiology and Immunology,Doylestown, PA 18901, alexander.karasev@jefferson.eduS FoulkeInstitute of Human Virology, University of Maryland Biotechnology Institute, Baltimore, MD 21202C WellensBiotechnology Foundation Laboratories at Thomas Jefferson University, Department of Microbiology and Immunology,Doylestown, PA 18901I ZwierzynskiBiotechnology Foundation Laboratories at Thomas Jefferson University, Department of Microbiology and Immunology,Doylestown, PA 18901R BaldwinBiotechnology Foundation Laboratories at Thomas Jefferson University, Department of Microbiology and Immunology,Doylestown, PA 18901This Article is brought to you for free and open access by the Jefferson Digital Commons. The Jefferson Digital Commons is a service of ThomasJefferson University's Center for Teaching and Learning (CTL). The Commons is a showcase for Jefferson books and journals, peer-reviewed scholarlypublications, unique historical collections from the University archives, and teaching tools. The Jefferson Digital Commons allows researchers andinterested readers anywhere in the world to learn about and keep up to date with Jefferson scholarship. This article has been accepted for inclusion inDepartment of Microbiology and Immunology Faculty Papers by an authorized administrator of the Jefferson Digital Commons. For moreinformation, please contact: JeffersonDigitalCommons@jefferson.edu.Recommended CitationKarasev, A V.; Foulke, S; Wellens, C; Zwierzynski, I; Baldwin, R; Koprowski, H; and Reitz, M S. Jr,"Orally Delivered, Plant-Produced Tat Protein Primes Mice For a Challenge DNA VaccineExpressing Tat" (2005). Department of Microbiology and Immunology Faculty Papers. Paper 11.http://jdc.jefferson.edu/mifp/11See next page for additional authorsLet us know how access to this document benefits youFollow this and additional works at: http://jdc.jefferson.edu/mifpPart of the Allergy and Immunology Commons, Infectious Disease Commons, and the MedicalGenetics CommonsAuthorsA V. Karasev, S Foulke, C Wellens, I Zwierzynski, R Baldwin, H Koprowski, and M S. Reitz JrThis presentation is available at Jefferson Digital Commons: http://jdc.jefferson.edu/mifp/11BioMed CentralPage 1 of 1(page number not for citation purposes)RetrovirologyOpen AccessOral presentationOrally Delivered, Plant-Produced Tat Protein Primes Mice For a Challenge DNA Vaccine Expressing TatA V Karasev*‡1, S Foulke2, C Wellens1, I Zwierzynski1, R Baldwin1, H Koprowski1 and MS Reitz Jr2Address: 1Biotechnology Foundation Laboratories at Thomas Jefferson University, Department of Microbiology and Immunology, Doylestown, PA 18901 and 2Institute of Human Virology, University of Maryland Biotechnology Institute, Baltimore, MD 21202Email: A V Karasev* - alexander.karasev@jefferson.edu* Corresponding author    ‡Presenting author    The Tat protein has been recently explored as a prospec-tive vaccine candidate against HIV-1 with broad, subtypenon-specific action. A truncated version of Tat()Tat) withthe basic loop, involved in immunosuppression, removedhas been previously demonstrated as efficacious as thefull-size Tat protein. We produced both full-size Tat andtruncated _Tat in plants, including one edible species –spinach, thus simultaneously addressing problems of aninexpensive Tat production and a direct delivery throughthe mucosal route. We tested this oral delivery route in amouse model. Tat and )Tat genes were assembled from aset of synthetic overlapping oligonucleotides, and subse-quently cloned into a plant virus-based expression vector.Codon optimization allows production of up to 300–500mg of Tat or )Tat antigen per 1 g of leaf tissue in spinach.Spinach plants inoculated with the Tat-producing con-structs were collected and fed to mice 7–14 days post inoc-ulation with or without mucosal adjuvants. Mice were fedwith the Tat-producing or control vector-inoculated spin-ach. After 3 voluntary feedings, 1 week apart, 1 g per mice,no differences were detected in the growth rate or behav-ior of the animals fed with these two types of spinach.None of the animals developed measurable Tat antibod-ies. Challenge DNA vaccination with a homologous Tat-expressing construct was performed using a gene gun. Fol-lowing DNA vaccination, however, mice previouslyreceiving oral Tat with cholera toxin as an adjuvant, devel-oped higher antibody titers to Tat than did the controls,with the titers peaking at four weeks post-vaccination.Thus, our data suggested that oral Tat primed for thedevelopment of Tat antibodies when mice were challenge-vaccinated with plasmid DNA for expression of Tat.from 2005 International Meeting of The Institute of Human VirologyBaltimore, USA, 29 August – 2 September 2005Published: 8 December 2005Retrovirology 2005, 2(Suppl 1):S67 doi:10.1186/1742-4690-2-S1-S67<supplement> <title> <p>2005 International Meeting of The Institute of Human Virology</p> </title> <note>Meeting abstracts – A single PDF containing all abstracts in this Supplement is available here. [link 'here' using 'a href' to: <url>http://www.biomedcentral.com/content/pdf/1742-4690-2-S1-full. df</url>]</not /supplement>

http://jdc.jefferson.edu/cgi/viewcontent.cgi?article=1011&context=mifp

Orally Delivered, Plant-Produced Tat Protein Primes Mice For a Challenge DNA Vaccine Expressing Tat

Abstract

Similar works

Full text

Available Versions

Name not available

Jefferson Digital Commons