Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/102186/1/aji12153.pd

Aronoff, David M.

Bourdonnay, Emilie

Fordyce, Krystle

Lewis, Casey

Peters‐golden, Marc

Rogers, Lisa M.

Serezani, Carlos H.

Thelen, Tennille

Xie, Jingli

Yu, Han

Zhang, Junyong

American Journal of Reproductive Immunology

PubMed

PROBLEM: Clostridium sordellii causes endometrial infections, but little is known regarding host defenses against this pathogen.
METHOD OF STUDY: We tested the hypothesis that the immunoregulatory lipid prostaglandin (PG) E2 suppresses human macrophage clearance of C. sordellii through receptor-induced increases in intracellular cyclic adenosine monophosphate (cAMP). The THP-1 macrophage cell line was used to quantify C. sordellii phagocytosis.
RESULTS: PGE2 increased cAMP levels, activated protein kinase A (PKA), and inhibited the class A scavenger receptor-dependent phagocytosis of C. sordellii. Activation of the EP2 and EP4 receptors increased intracellular cAMP and inhibited phagocytosis, with evidence favoring a more important role for EP4 over EP2. This was supported by EP receptor expression data and the use of pharmacological receptor antagonists. In addition, the PKA isoform RI appeared to be more important than RII in mediating the suppression of ingestion of C. sordellii.
CONCLUSION: The endogenous lipid mediator PGE2 impairs human innate immune responses against C. sordellii

Peters-Golden, Marc

IUPUIScholarWorks

EP4 and EP2 receptor activation of protein kinase A by prostaglandin E2 impairs macrophage phagocytosis of Clostridium sordellii

Deep Blue Documents

EP4 and EP2 Receptor Activation of Protein Kinase A by Prostaglandin E 2  Impairs Macrophage Phagocytosis of  Clostridium sordellii

Lisa M. Rogers

Tennille Thelen

Krystle Fordyce

Emilie Bourdonnay

Casey Lewis

Han Yu

Junyong Zhang

Jingli Xie

Carlos H. Serezani

Marc Peters-Golden

David M. Aronoff

Crossref

EP4 and EP2 Receptor Activation of Protein Kinase A by Prostaglandin E<sub>2</sub>Impairs Macrophage Phagocytosis of<i>Clostridium sordellii</i>

https://deepblue.lib.umich.edu/bitstream/2027.42/102186/1/aji12153.pdf

EP4 and EP2 Receptor Activation of Protein Kinase A by Prostaglandin E 2 Impairs Macrophage Phagocytosis of Clostridium sordellii

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