Reif, Raymond

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EXCLI Journal 2014;13:1283-1285 – ISSN 1611-2156 Received: December 10, 2014, accepted: December 13, 2014, published: December 15, 2014   1283 Letter to the editor: THE BODY-ON-A-CHIP CONCEPT: POSSIBILITIES AND LIMITATIONS  Raymond Reif  Leibniz Institut für Arbeitsforschung an der TU Dortmund,  Leibniz Research Centre for Working Environment and Human Factors (IfADo),  Ardeystrasse 67, 44139 Dortmund, Germany; reif@ifado.de   Dear Editor,  Recently, Frey et al. (2014) have established a reconfigurable microfluidic platform to study multi-tissue interactions. This platform contains multiple spheroids of different cell types in hanging drops. The hanging drops are connected by microfluidic networks. The path of the liquid flow through the hanging drops is precisely controlled and offers the possibility to perfuse them either sequently or parallelized. For example culture media may first pass a hanging drop with a liver spheroid and subsequently pass kidney, heart, bone marrow, or neu-ronal tissues (Frey et al., 2014). Currently, many groups work on the optimization of ‘body-on-a-chip’ systems (Kelm and Marchan, 2014; Sung et al., 2014; Williamson et al., 2013). Currently, ‘organ-on-a-chip’ concepts are developed for many tissues including heart (Zwei-gerdt et al., 2014; Agarwal et al., 2013), kidney (Jang et al., 2013), lung (Punde et al., 2014; Weis et al., 2013; Huh et al., 2012) and intestine (Esch et al., 2014, 2012). Years before the ‘body-on-a-chip movement’ much work has been invested in the optimization of three dimen-sional culture systems (Xie et al., 2006; Marquette et al., 2007; De Kock et al., 2011; Teich-mann et al., 2014; Ramaiahgari et al., 2014). Among the easiest and most efficient methods of 3D culture are the collagen sandwich technique, where cells are cultivated between two layers of soft gel collagen (Schug et al., 2008; 2013; O’Brien, 2006) or cell spheroids which can be generated by hanging drop cultures (Messner et al., 2013; Godoy et al., 2013). Today organo-typical in vitro systems are frequently used to study mechanisms of toxicity, particularly in the fields of hepatotoxicity (Schyschka et al., 2013; Rodriques et al., 2013; Watzek et al., 2013; Ilkavets, 2013), nephrotoxicity (Limonciel et al., 2012; Jennings et al., 2012) and de-velopmental toxicity (Bolt, 2013; Balmer et al., 2014; Zimmer et al., 2014; Stern et al., 2014; Krug et al., 2013a, b). These organotypical in vitro systems are now used in ‘body-on-a-chip’ devices if they can be transferred to cell culture microdevices.  Nevertheless, despite of recent progress in ‘body-on-a-chip’ research it is clear that this concept is still in its infancy. For example, the overall quality of the ‘body-on-a-chip’ is lim-ited by the quality of its ‘microorgans’. Although multiple publications claim that three di-mensional (3D) culture systems represent higher levels of tissue organization, this is certainly not correct. Correct would be that 3D culture systems represent some aspects of real tissue but many tissue functions are not represented. For example liver microtissues establish bile cana-liculi between the hepatocytes. Therefore, these model systems may be used to study excre-tion of compounds from hepatocytes into bile canaliculi. However, liver microtissues current-ly do not establish sinusoids, the liver’s microvessels (Hammad et al., 2014). This causes nu-merous differences to real liver tissue. For example the sinusoidal tissue unit is not correctly established. Liver sinusoidal endothelial cells (LSECs) with Kupffer cells at their luminal and stellate cells at the parenchymal side are responsible for numerous mechanisms in toxicology, EXCLI Journal 2014;13:1283-1285 – ISSN 1611-2156 Received: December 10, 2014, accepted: December 13, 2014, published: December 15, 2014   1284 ranging from interactions with circulating immune cells to pathogenesis of liver fibrosis. Ob-viously, this sinusoidal tissue unit is not correctly recapitulated by the currently available arti-ficial microtissues. Similar critical limitations could be described for microtissues represent-ing other organs. In conclusion, microfluidics offer the prospect to establish complex physio-logical scenarios under accurately ‘reproducible in vitro conditions’. However, the hunt for a ‘body-on-a-chip’ or even an ‘organ-on-a-chip’ that really deserves this name has only just be-gun.   REFERENCES Agarwal A, Goss JA, Cho A, McCain ML, Parker KK. 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The body-on-a-chip concept: possibilities and limitations

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The body-on-a-chip concept: possibilities and limitations

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