Our results reveal that the Feo/Klp3A complex is necessary for Polo recruitment to the spindle midzone. A similar finding has also been recently reported in mammalian cells [1], suggesting that this basic mechanism has been conserved during evolution, albeit with some differences. Finally, since cleavage furrow formation and ingression are unaffected following feo RNAi, our data imply that Polo recruitment to the central spindle is not required for furrowing, but some other aspect of cytokinesis

Archambault, Vincent

D'Avino, Pier Paolo

Glover, David M.

Laue, Ernest

Lilley, Kathryn S.

Przewloka, Marcin

Zhang, Wei

PubMed

BackgroundPolo-like kinases control multiple events during cell division, including mitotic entry, centrosome organization, spindle formation, chromosome segregation and cytokinesis. Their roles during cytokinesis, however, are not well understood because the requirement of these kinases during early stages of mitosis complicates the study of their functions after anaphase onset.Methodology/principal findingsWe used time-lapse microscopy to analyze the dynamics of Polo::GFP in Drosophila tissue culture cells during mitosis. After anaphase onset, Polo::GFP concentrated at the spindle midzone, but also diffused along the entire length of the central spindle. Using RNA interference we demonstrate that the microtubule-associated proteins Feo and Klp3A are required for Polo recruitment to the spindle midzone, but not the kinesin Pavarotti as previously thought. Moreover, we show that Feo and Klp3A form a complex and that Polo co-localizes with both proteins during cytokinesis.Conclusion/significanceOur results reveal that the Feo/Klp3A complex is necessary for Polo recruitment to the spindle midzone. A similar finding has also been recently reported in mammalian cells [1], suggesting that this basic mechanism has been conserved during evolution, albeit with some differences. Finally, since cleavage furrow formation and ingression are unaffected following feo RNAi, our data imply that Polo recruitment to the central spindle is not required for furrowing, but some other aspect of cytokinesis

Pier Paolo D'Avino

Vincent Archambault

Marcin R Przewloka

Wei Zhang

Kathryn S Lilley

Ernest Laue

David M Glover

Directory of Open Access Journals

PLoS ONE

Recruitment of Polo kinase to the spindle midzone during cytokinesis requires the Feo/Klp3A complex.

Background: Polo-like kinases control multiple events during cell division, including mitotic entry, centrosome organization, spindle formation, chromosome segregation and cytokinesis. Their roles during cytokinesis, however, are not well understood because the requirement of these kinases during early stages of mitosis complicates the study of their functions after anaphase onset. 



Methodology/Principal Findings: We used time-lapse microscopy to analyze the dynamics of Polo::GFP in Drosophila tissue culture cells during mitosis. After anaphase onset, Polo::GFP concentrated at the spindle midzone, but also diffused along the entire length of the central spindle. Using RNA interference we demonstrate that the microtubule-associated proteins Feo and Klp3A are required for Polo recruitment to the spindle midzone, but not the kinesin Pavarotti as previously thought. Moreover, we show that Feo and Klp3A form a complex and that Polo co-localizes with both proteins during cytokinesis. 



Conclusion/Significance: Our results reveal that the Feo/Klp3A complex is necessary for Polo recruitment to the spindle midzone. A similar finding has also been recently reported in mammalian cells [1], suggesting that this basic mechanism has been conserved during evolution, albeit with some differences. Finally, since cleavage furrow formation and ingression are unaffected following feo RNAi, our data imply that Polo recruitment to the central spindle is not required for furrowing, but some other aspect of cytokinesis

Przewloka, Marcin R.

Caltech Authors - Main

Recruitment of Polo Kinase to the Spindle Midzone during Cytokinesis Requires the Feo/Klp3A Complex

Southampton (e-Prints Soton)

Recruitment of Polo kinase to the spindle midzone during cytokinesis requires the Feo/Klp3A complex

A nematode kinesin required for cleavage furrow advancement.

A plus-end-directed motor enzyme that moves antiparallel microtubules in vitro localizes to the interzone of mitotic spindles.

A RhoGEF and Rho family GTPase-activating protein complex links the contractile ring to cortical microtubules at the onset of cytokinesis.

Cell cycle regulation of central spindle assembly.

Cell cycle-dependent translocation of PRC1 on the spindle by Kif4 is essential for midzone formation and cytokinesis.

Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity.

Chemical genetics reveals the requirement for Polo-like kinase 1 activity in positioning RhoA and triggering cytokinesis in human cells.

Choice of Plk1 docking partners during mitosis and cytokinesis is controlled by the activation state of Cdk1.

Cleavage furrow formation and ingression during animal cytokinesis: a microtubule legacy.

Cooperation between mitotic kinesins controls the late stages of cytokinesis.

Cytokinesis and midzone microtubule organization in Caenorhabditis elegans require the kinesin-like protein ZEN-4.

Cytokinesis: welcome to the Rho zone.

Domains of the Pavarotti kinesin-like protein that direct its subcellular distribution: effects of mislocalisation on the tubulin and actin cytoskeleton during Drosophila oogenesis.

Drosophila Polo kinase is required for cytokinesis.

Essential roles of KIF4 and its binding partner PRC1 in organized central spindle midzone formation.

Feo, the Drosophila homolog of PRC1, is required for central-spindle formation and cytokinesis.

Heterologous expression of mammalian Plk1 in Drosophila reveals divergence from Polo during late mitosis.

Molecular interactions of Pololike-kinase 1 with the mitotic kinesin-like protein CHO1/MKLP-1.

Multiple protein phosphatases are required for mitosis in Drosophila.

Mutations in sticky lead to defective organization of the contractile ring during cytokinesis and are enhanced by Rho and suppressed by Rac.

pavarotti encodes a kinesin-like protein required to organize the central spindle and contractile ring for cytokinesis.

Phosphorylation of ZEN-4/MKLP1 by Aurora B regulates completion of cytokinesis.

Plk is an M-phase-specific protein kinase and interacts with a kinesin-like protein, CHO1/MKLP-1.

Polo kinase and progression through M phase in Drosophila: a perspective from the spindle poles.

Polo-like kinase Cdc5 controls the local activation of Rho1 to promote cytokinesis.

Polo-like kinase controls vertebrate spindle elongation and cytokinesis.

Polo-like kinases and the orchestration of cell division.

polo, a mitotic mutant of Drosophila displaying abnormal spindle poles.

PRC1 is a microtubule binding and bundling protein essential to maintain the mitotic spindle midzone.

Site selection for the cleavage furrow at cytokinesis.

SPD-1 is required for the formation of the spindle midzone but is not essential for the completion of cytokinesis in C. elegans embryos.

The cellular geography of aurora kinases.

The Drosophila kinesin-like protein KLP3A is a midbody component required for central spindle assembly and initiation of cytokinesis.

The Drosophila POLO kinase localises to multiple compartments of the mitotic apparatus and is required for the phosphorylation of MPM2 reactive epitopes.

The roles of microtubule-based motor proteins in mitosis: comprehensive RNAi analysis in the Drosophila S2 cell line.

https://figshare.com/articles/Recruitment_of_Polo_Kinase_to_the_Spindle_Midzone_during_Cytokinesis_Requires_the_Feo_Klp3A_Complex/152379

Recruitment of Polo kinase to the spindle midzone during cytokinesis requires the Feo/Klp3A complex

Abstract

Similar works

Full text

Available Versions

Directory of Open Access Journals

Caltech Authors - Main

Southampton (e-Prints Soton)