Potential of Sea Cucumber Rivet Red Extract (Holothuria leucospilota) As Antibacterial MDR (Multi Drug Resistant)

Abstract

Resistance of microorganisms to antibiotics is a serious problem in the treatment of infections of this period, it is necessary to search new bioactive compounds that can be used as an antibiotic. Secondary metabolites found in marine organisms is one of the alternative materials discovery of new antibiotics. Such animals as sea cucumbers potential producers of bioactive compounds of secondary metabolism is sea cucumber. Rivet sea cucumbers or Holothuria leucospilota is a lot of sea cucumbers found in waters Bandengan, Jepara, but not much utilization. The purpose of the study was to determine the antibacterial activity of the extract fractions rivet red sea cucumber (H. leucospilota) against MDR bacteria and compounds that have potential as antibacterial MDR. Analysis of samples of sea cucumbers includes extraction, fractionation, and analysis of bacterial sensitivity test Gas Chromatography-Mass Spectrometer (GC-MS), the extraction process is carried out by solid-liquid extraction method (solid-liquid). Fractionation is done with Open Column Chromatography (KKT). Test sensitivity of bacteria using the agar diffusion method according to the Kirby-Bauer. The results showed that the extract of Holothuria leucospilota has 7 fractions active against MDR bacteria Klebsiella sp and Enterobacter 10. The average value of inhibition zone is highest in fraction III with concentration of 80 ug / disk for each type of bacteria Klebsiella sp and Enterobacter 10 was 11.87 ± 0.90 mm and 11.49 ± 0.86 mm. The results of GC-MS analysis showed that the fraction IV containing the compound 2-methyl butanoic; 2-butoxy ethanol; 3,5,5 trimethyl 2-cyclohexane-1; propandiat phenyl and 2-methoxy-4-(2-propenyl) or eugenol