The spermatozoa quality of
goramy after 2 d sub-zero freezing was examined. The quality of spermatozoa examined included motility,
viability, and abnormality. We aimed to determine the optimum concentration of glycerol protecting
spermatozoa during preservation. We used 0%, 1%, 3%, 5%, 7%, and 9% of glycerol, respectively. Sperms were
diluted by the combination of glycerol and fish ringer (1 part of sperm + 3 part of solvent). The dilute sperms
were then equiliberated at 4°C for 45 min, and were freezed at -34°C for 2 d. Thawing was then carried out at
30°C for 2 min. Based on Dunnet test, 5% of glycerol was the optimum concentration maintaining spermatozoa
motility (75.95±4.76)%
