本文以正十九碳酸（C19：0）作内标，用HCI-CH3OH对海洋微藻（三角褐指藻）进行抽提酯化后做毛细管气相色谱分析。方法的重现性各脂肪酸的相对偏差为0.3%~11.6%，回收率为85.7%~103.3%，仪器稳定性的相对偏差为0.2%~3.1%。A capillary column gas chromatographic technique For the determination of microalgae ( phaeodactylum tricornutum )Fatty acids has been developed.The Fatty acid extraction and esteriFication is Finished in one step.Eleven kinds of Fatty acid methyl esters have been identiFied.The CV is less than 7% except C 22:5ω3 and the recovery rate 85.7～103.3%.Thus a rapid,convenient,reliable and microalytical method is established For the study on developing and making use of microalgae
