Brassac, Jonathan

Degenhardt, Jörg

Mondal, Prodyut

Otto, Lars-Gernot

Preiss, Susanne

Sharbel, Timothy Francis

English

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Use of genotyping by sequencing (GBS) in chamomile (Matricaria recutita L.) to enhance breeding

JKI Open Journal Systems (Julius Kühn-Institut)

6th International Symposium Breeding Research on Medicinal and Aromatic Plants, BREEDMAP 6, Quedlinburg, Germany, June 19-23, 2016  Julius-Kühn-Archiv, 453, 2016 17 ASL 3: Use of genotyping by sequencing (GBS) in chamomile (Matricaria recutita L.) to enhance breeding Lars-Gernot Otto1, Jonathan Brassac1, Prodyut Mondal2, Susanne Preiss2, Jörg Degenhardt2 , Timothy Francis Sharbel1, 3 1  Apomixis Research Group, Department Plant Breeding Research, Leibniz Institute for Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, D-06466 Stadt Seeland OT Gatersleben, Germany; E-mail: ottolg@web.de (corresponding author) 2 Research Group of Pharmaceutical Biotechnology, Institute of Pharmacy, Martin-Luther University Halle-Wittenberg, Hoher Weg 8, D-06120 Halle (Saale), Germany 3 Global Institute for Food Security, 110 Gymnasium Place, University of Saskatchewan, Saskatoon, Saskatche-wan, Canada S7N 4J8 DOI 10.5073/jka.2016.453.004  Abstract German chamomile (Matricaria recutita L.) is an important medicinal plant with a wide range of medical uses. Genotyping by Sequencing (GBS) was performed for this non-model organism on a panel of 33 different origins (varieties, populations, accessions; in total 91 plants with 2-4 plants per origin), and for 2 accessions of M. discoidea as outgroup. High-quality SNP identification (single nucleotide polymorphisms) was done using the pyRAD pipeline, and genetic diversity was ana-lysed with STRUCTURE and CLC Genomics Workbench 8.5. The analysis revealed one group with low genetic differentiation (14 origins with 39 plants), whereas clear differences could be identified between the remaining 19 origins (52 plants). Single plants of the same origin were mostly genetically similar, although elevated genetic diversity could be identified between single plants of 13 origins.  Phenotypic traits (flowering time, chemical compounds, powdery mildew infection) and ploidy were also measured. Ongoing work includes the identification of associations between genetic data (SNPs) and these traits. These data will be used to improve the exploitation of genetic re-sources in chamomile (e.g. utilization of heterosis, generation of cytoplasmic male sterility, facilita-tion of breeding of varieties with improved traits). To summarize, we have applied next generation genomics methods to medicinal plants as a basis to improve the breeding process. The efforts to perform such an analysis are manageable, espe-cially regarding the decreasing costs and increasing availability of bioinformatic pipelines for the GBS-analysis.    

https://ojs.openagrar.de/index.php/JKA/article/download/6472/6074

Use of genotyping by sequencing (GBS) in chamomile (Matricaria recutita L.) to enhance breeding

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