Projection structure of the surface layer of methanoplanus limicola at 10-angstrom resolution obtained by electron cryomicroscopy

Abstract

The projection structure of the surface protein layer of the archaeon Methanoplanus limicola was analysed by electron microscopy and correlation averaging techniques to a resolution of about 1 nm. For electron microscopy the 2D crystalline protein layer was embedded in aurothioglucose. in order to minimize beam damage, specimen drift, charging and other effects degrading the images, cryomicroscopy at -120 degrees C in combination with spot-scan illumination technique with spot sizes in the range from 200 to 1000 nm was used for recording the images. (C) 1996 Academic Press, Inc. [References: 16