Bhin, J

Hong, KS

Hwang, D

Jeong, HS

Jung, HS

Kim, C

Kim, JS

Kim, KS

Shin, JO

Molecules and Cells

PubMed

HKU Scholars Hub

PGC-Enriched miRNAs Control Germ Cell Development

Non-coding microRNAs (miRNAs) regulate the translation of target messenger RNAs (mRNAs) involved in the growth and development of a variety of cells, including primordial germ cells (PGCs) which play an essential role in germ cell development. However, the target mRNAs and the regulatory networks influenced by miRNAs in PGCs remain unc lear. Here, we demonstrate a novel miRNAs control PGC development through targeting mRNAs involved in various cellular pathways. We reveal the PGC-enriched expression patterns of nine miRNAs, including miR-10b, -18a, -93, - 106b, -126-3p, -127, -181a, -181b, and -301, using miRNA expression analysis along with mRNA microarray analysi sin PGCs, embryonic gonads, and postnatal testes. These miRNAs are highly expressed in PGCs, as demonstrated by Northern blotting, miRNA in situ hybridization assay, and miRNA qPCR analysis. This integrative study utilizing mRNA microarray analysis and miRNA target prediction demonstrates the regulatory networks through whic h these miRNAs regulate their potential target genes during PGC development. The elucidated networks of miRNAs disclose a coordinated molecular mechanism by which these miRNAs regulate distinct cellular pathways in PGCs that determine germ cell development. © The Korean Society for Molecular and Cellular Biology. All rights reserved

Bhin, Jinhyuk

Jeong, Hoe-Su

Kim, Jong Soo

Shin, Jeong Oh

Hong, Ki Sung

Jung, Han-Sung

Kim, Changhoon

Hwang, Daehee

Kim, Kye-Seong

DGIST Library Institutional Repository

http://dx.doi.org/10.14348/molcells.2015.0146

PGC-Enriched miRNAs Control Germ Cell Development

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HKU Scholars Hub

DGIST Library Institutional Repository