CARNEIRO, N. P.

GANDER, E. S.

LOPES, M. A.

MARCELLINO, L. H.

PAIVA, E.

PORTUGAL, R. S.

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Plant & Animal Genome VIII ConferenceCARNE I RO,N.P.2000Town & Country Hotel, San Diego, CA, January 9-12, 2000.CLONING OF PUTATIVE SEED STORAGE PROTEIN GENES OF PEARL MILLET(Pennisetum glaucum)1 EMBRAPNCNPMS Rodovia MG 424, Km 65 Sete Lagoas - Minas Gerais Brazil35701-9702 EMBRAPNCENARGEN SAIN - Parque Rural-Final Av W5 Norte Caixa Postal 02372 Brasilia - DFBrazil - 70770-900Gene prospection is an important strategy ofbiotechnology for the identification of genes,promoters and regulatory sequences for the potential use in the improvement ofbiologicalprocesses in plants. In this work we report a prospection study on storage genes and promoters inPearl Millet. Better knowledge about storage polypeptides and their genes in graminea species canlead to the identification of genes potentially useful for the manipulation of grain quality ineconomically important cereal species. In previous studies at EMBRAP NCNPMS, comparativeanalyses of seed proteins of maize, sorghum, pearI millet, teosinte and tripsacum showed that thereare similarities amongst the polypeptides of these species. These results were reinforced byimmunological analyses ("westem blotting") with polyc1onal antibodies raised against maizeproteins (alpha-, beta- and gamma-zeins), that showed cross-reactions between polypeptides withequivalent Mr and solubility in the species analysed. Based on these similarities we initiated theisolation of storage protein genes in a Pearl Millet genomic library using cDNA probes coding foralpha-, beta-, gamma- and delta-zeins from maize. Putative storage protein genomic clones havebeen isolated and they have been characterized by restriction enzymes. The results will help todirect our work on identification, cloning and characterization of genes and regulatory sequencespotentially useful for the improvement of cereal grain quality through genetic engineering.nttp: IIwww.mtl-pag.orgtpagt'6/aOstractSlpag'6u'6:>.nunl lU/UI/LUUU

Cloning of putative seed storage protein genes of pearl millet (Pennisetum glaucum).

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