This work is part of the Strategies and Actions for Biodiversity Conservation of Atlantic Forest Project supported by Critical Ecosystem Partnership Fund and was developed in Rio de Janeiro State, Brazil, which has only 20,33% of its area covered by vegetation, in a fragmented landscape The objective is to identify areas in Rio de Janeiro state that the conservation is more propitious or critical. For this, it was applied spatial analysis to know the distribution of vegetation patches and the connection possibilities based on the diversity of the land use matrix and its permeability. It was observed that 25% of the remaining vegetation of the state is isolated of any reserve and the great part of them is in the Semi-deciduous seasonal forest region, where the conditions are more critical due to the smaller size ofpatches and their isolation
