Glaucoma, a sever neurodegenerative ocular disease, is one of the leading causes of blindness worldwide. An increased intraocular pressure (IOP) is regarded as its main risk factor. The IOP is generated in the anterior eye due to a resistance to the outflow of the aqueous humor through the trabecular outflow pathway. Recently, connective tissue growth factor (CTGF) was identified to increase the resistance to aqueous humor outflow by enhancing the extracellular matrix deposition and contractility of cells in the trabecular meshwork (TM). Post transcriptional gene silencing using small interfering RNA (siRNA) is an elegant strategy to reduce elevated levels of CTGF and consequently IOP. Nevertheless, delivering nucleic acids of this type remains a challenge. This work describes the development of hyaluronic acid functionalized layer-by-layer nanoparticles for the delivery of siRNA to the TM
