BALDI, F.

CASTRO, L. M. de

LOPES, F. B.

MAGNABOSCO, C. de U.

PERIPOLLI, E.

SILVA, R. M. de O.

English

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Genome wide association study for beef tenderness in Polled Nellore cattle. Elisa Peripolli1,3; Rafael M. de O. Silva1; Letícia M. de Castro2; Fernando B. Lopes2;Claudio de U. Magnabosco2, and Fernando Baldi11FCAV-UNESP, Jaboticabal,  SP, Brazil,  2Embrapa Cerrados,  Planaltina,  DF,  Brazil,3CAPES/EMBRAPA.The aim of this study was to identify genomic regions associated with meat tendernessin a Polled Nellore cattle population. The dataset was provided by the Guaporé PecuáriaS/A (OB Ranch) and Embrapa Cerrados, and contained 1102 animals in which 436 ofthem had records of tenderness measured approximately at 2 years of age. A total of 609animals  were  genotyped  either  with  BovineHD  BeadChip  (Illumina  Inc.)  or  GGP-Indicus  HD (GeneSeek  Inc.),  remaining  600  genotyped  animals  and 375,668  SNPsavailable for analysis after the genomic quality control. SNP solutions were estimatedby genome-wide association using a single-step genomic BLUP procedure (ssGWAS).Variance components were estimated for tenderness traits using an animal model underBayesian inference. The model included fixed effects of sex, slaughter group, age atslaughter (covariate), and direct animal random effect. Variances were calculated forwindows of 1 Mb. The regions that accounted for more than 1% of additive geneticvariance were used to search for candidate genes. The Map Viewer of bovine genome(UMD3.1 version) and Ensembl Genome Brower were used for gene identification, andits biological function was identified on GeneCards. The results indicated a total of 19windows explaining more than 1% of the genetic variance for tenderness. Among thosewindows, genes related to intracellular lipid receptors and fatty acid binding proteinswere identified,  such as  OSBPL1A (BTA24) and FABP7 (BTA9),  respectively.  Thegene CAPZA1 (BTA3) which regulates  growth of  actin  filament  and that  had beenpreviously associated with leaf fat in swine was also identified. A gene which has beenpreviously related to marbling content in cattle (TAGLN2) was found on BTA3. Thestudy  revealed  genes  related  to  muscle  weakness,  such  as  KLHL41  and  CASQ1.Calcium ion binding and calcium-dependent phospholipid binding gene (MCTP1) wasobserved on BTA7. The MYO3B gene that encodes class III myosins was found onBTA2. The CAST gene was found close to the region with the highest genetic variancefor tenderness. The CAST gene is considered important functional candidate for meattenderness in livestock. Results found in this study should help to better understand thegenetic and physiologic mechanism associated with tenderness in beef cattle.Key words: Meat Quality, Candidate gene, LivestockInternational Meeting of Advances in Animal ScienceISSN: 2448-4385Vol. 1 - 2016Galoá {Este trabalho foi publicado utilizando o Galoá Proceedings

Genome wide association study for beef tenderness in Polled Nellore cattle.

http://www.alice.cnptia.embrapa.br/bitstream/doc/1048593/1/35474.pdf

Genome wide association study for beef tenderness in Polled Nellore cattle.

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