BIZZO, H. R.

MARRIOTT, P. J.

WONG, Y. F.

YAN, D.

English

Repository Open Access to Scientific Information from Embrapa

RAPID PORTABLE GC-MS ANALYSIS FOR ON-SITE SCREENING OFPHYTOCHEMICAL LEAF OILS IN AN AUSTRALIAN NATIVE BOTANIC GARDENYong Foo Wong1, Dandan Yan2,3, Humberto Bizzo4, Philip J. Marriott11 Monash University, Wellington Rd, 3800 Clayton, Australia2 School of Physical Sciences - University of Tasmania, Sandy Point Road, 7001 Hobart, Australia3 School of Chemistry - Monash University, Wellington Road, 3800 Clayton, Australia4 Embrapa, Av. das Américas, 29501, 23020-470 Rio de Janeiro, BrazilThe plant volatile compound fraction is an important marker that is diagnostic of plant origin,phenotype, and variety/cultivar. Numerous analytical arrangements (comprising both samplingand instrumental techniques) have been reported for this task, aiming to identify the best trade-off between extraction, separation and analysis time. In this study, we demonstrated rapid on-site screening of volatiles emissions from leaves of living plants, using headspace solid-phasemicroextraction (HS-SPME) with a portable low-thermal mass gas chromatography (GC)method, equipped with an ion trap mass spectrometer (ITMS). The study was conducted at theRoyal Botanical Garden, Cranbourne, with sampling located in the Peppermint Garden. Twelvedesignated plants in the families of Myrtaceae and Rutaceae were chosen for this field study.SPME is recommended for sample introduction with this instrument, and is well-suited to thepresent analysis; leaves were collected into a 20 mL vial, equilibrated for 10 min (at prevailingenvironmental temperature), SPME sampling onto a PDMS/DVB – coated fibre for 2 min, anddesorption at 270 °C in the splitless-mode injector for ca. 10 s. The portable GC-MS analysiscomprised on-board battery operation providing at least 4 h operation, and an external size DHe cylinder. A MXT-5 column of dimension 5 m × 0.1 mm I.D. × 0.4 µm df was resistivelyheated. The short SPME sampling time combined with fast separation (2 °C/s temperatureprogram rate; total run time of 3 min), provided a near-real-time measurement of leaf volatilesreleased from the plant. Results were further processed by “chromatographic fingerprinting”,and “template matching” based on MS fragmentation pattern similarity criteria and retentionindices. Unsupervised multivariate analysis was performed to improve effectiveness andspecificity for the classification of different plant volatiles. The combination of HS-SPME andthe portable GC-ITMS system proved to be a promising technique for rapid chemical expressionof the plant volatiles genotype.I.09285

Rapid portable GC-MS analysis for on-site screening of phytochemical leaf oils in an Australian Native Botanic Garden.

https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/1046603/1/40thISCCP285.pdf

Rapid portable GC-MS analysis for on-site screening of phytochemical leaf oils in an Australian Native Botanic Garden.

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