ALVES, A. A. C.

BOMFIM, C. A.

FONTENELLE, M. R.

MENDES, L. S.

VALE, H. M. M. do

ZANDONADI, D. B.

English

Repository Open Access to Scientific Information from Embrapa

 962-2 - PLANT HORMONE INDOLE-3-ACETIC ACID CONTENT IN ORGANICLIQUID BIOFERTILIZER Lucas Soares Mendes1, Catharine Abreu Bomfim2, Anaíse Amaral Coelho Alves3, Mariana Rodrigues Fontenelle4, Helson Mário Martins do Vale2, Daniel Basílio Zandonadi4 1 ICESP/PROMOVE - Promove of Brasília, 2 UNB - University of Brasília, 3 UCB - Catholic University of Brasília, 4 CNPH - Embrapa Vegetables Área: Microbiologia do Solo Sub Área: Microbiologia de Solos em Ambientes Agrícolas Palavras-chave:  auxin, organic agriculture, bacteria, fungi, actinomycetes Data de apresentação: 21/10/2015         Painel número:093 Plant hormones are molecules essential for normal plant growth and development associated to myriad of processes in plant and microorganisms. Biofertilizers are products that contain living microorganisms that when in contact with plants enhance growth or produces other beneficial response. The two objectives of this research were to evaluate a solid phase extraction procedure for extraction ofindole-3-acetic acid (IAA) from a liquid biofertilizer developed by Embrapa Hortaliças and to evaluate the changes in this plant hormone level at three different periods time(10, 20 and 30 days of fermentation) and the general group of microorganism associated. The method of preparation and analysis of IAA from biofertilizer was performed by centrifugation, followed  by pH adjustment  and solvent extraction and a further cleaning by cartridge containing mixed-mode anion-exchange  and reverse-phase sorbents.A nucleosil C18 column was  used followed by fluorimetric detection  (λEX=  280/λEM=350  nm)byReversed Phase High Performance Liquid Chromatography (RP-HPLC)   determination. The linear regression equation of calibration curve (P < 0.0001) had ther 2= 0.995 and the limit of detection was below 0.06 μg mL-1. The IAA amount at 10, 20 and 30 days was 0.57, 0.32 and 0.15μg mL-1, respectively. Bothtotal number of bacteria and actinomycetes were higher at day 10, coinciding with the higher IAA production. The use of biofertilizers with potential biostimulant activity (IAA activity) in agriculture may help enhance plant rooting and productivity as a result. This is particularly important for low input agriculture such as organic farming.   Key-words: auxin, organic agriculture, bacteria, fungi, actinomycetes   Development agency: EMBRAPA 

Plant hormone indole-3-acetic acid content in organic liquid biofertilizer.

http://www.alice.cnptia.embrapa.br/bitstream/doc/1031960/1/962.pdf

Plant hormone indole-3-acetic acid content in organic liquid biofertilizer.

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