FRANÇA, B. N.

SOUSA, S. M. de

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 Analysis of Phosphorus-Starvation Tolerance 1 cis-acting regulatory DNA elements from rice, maize and sorghum  Bárbara França Negri1,2, Sylvia Morais de Sousa1.  1Embrapa Milho e Sorgo, Sete Lagoas  - MG  2Federal University of São João Del-Rei - UFSJ, São João Del-Rei - MG   Low soil phosphorus (P) availability is a major constraint for crop production in tropical regions and changes in root morphology are key strategies for plants to enhance P acquisition. Phosphorus-Starvation Tolerance 1 (OsPstol1) is a gene responsible to enhance root surface, P acquisition and grain yield in rice under P deficiency. Multiple Pstol1 homologs in sorghum also play a role in the root morphology and architecture, leading to grain yield gain under low P availability. In maize, candidate genes were pointed out as Pstol1 homologs based on Quantitative Trait Loci co-localization with root and P efficiency traits and gene expression. In the present study, we aimed to identify in silico the non-coding region of Pstol1 gene from rice and their homologs in maize and sorghum. The upstream region (-1 to -2039 pb) was analyzed using SIGNALSCAN program provided by NEW PLACE database in order to identify their cis-regulatory elements (CREs). Using this approach, 175 types of CRE were identified and 29 CREs were common among the three species. Some of these CREs such as ARR1AT, CAATBOX1, CACTFTPPCA1, DOFCOREZM and WRK710S elements were also identified in all rice Phosphate Transporter genes. These elements have between 6 and 39 duplications in each cis-regulatory sequence. In addition, each Pstol1 gene has typical CREs that can only be found in the respective genes. These results shed light on the elusive path of signaling Pstol1; however, more studies are required to comprehend the pathway leading to the activation of Pstol1 genes.  Supported by: Embrapa, Fapemig, CNPq and GCP. 0255

Analysis of phosphorus-starvation tolerance 1 cis-acting regulatory DNA elements from rice, maize and sorghum.

http://www.alice.cnptia.embrapa.br/bitstream/doc/1029265/1/Analysisphosphorus.pdf

Analysis of phosphorus-starvation tolerance 1 cis-acting regulatory DNA elements from rice, maize and sorghum.

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