FIM JUNIOR, G. A.

LOPES, N. S. S.

PILON, L. E.

VASO, C. O.

ZAFALON, L. F.

Portuguese

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Title: SENSITIVITY TO ANTIMICROBIAL AGENTS IN DIFFERENT SPECIES OF COAGULASE-NEGATIVE STAPHYLOCOCCI ISOLATED IN SHEEP'S MILK  Author:  Vaso, C.O.¹; Fim Júnior, G. A.², Lopes, N. S. S.¹, Pilon, L. E.2, Zafalon, L. F.3 Institution: ¹ UNICEP – Centro Universitário Central Paulista (Rua Miguel Petroni, Vila Alpes, 5111 - 13563-479 - São Carlos, SP); 2 UNESP – Universidade Estadual Paulista (Via acesso Prof. Paulo Donato Castellan - Vila Industrial - 14884-900 - Jaboticabal, SP); ³ Embrapa Pecuária Sudeste (Rodovia Washington Luiz, Km 234 - Caixa Postal: 339 - 13560-970 - São Carlos, SP).  Abstract:  Mastitis is an inflammation of the mammary gland and may cause microbiological and physical-chemical changes in milk and of glandular breast tissue, interfering in weight gain of the lamb. The objective of the study was to investigate the antimicrobial sensitivity coagulase-negative Staphylococci (CNS) isolated of sheep’s half mammary. The samples of milk were taken from a flock located in a center region in State of São Paulo, formed by animals of Santa Inês, Texel, Ile-de-France and Dorper breed. The preliminary diagnostic of mastitis was realized by the California Mastitis Test (CMT) and the physical exam of the half mammary. After the antisepsis of the ostium papillary with cotton and alcohol 70%, milk samples of each mammary were taken in sterilized tubes, duplicated, and sent to  Embrapa Pecuária Sudeste in Sao Carlos, SP for microbiologic exams. The biological identification followed the morphotinctorial and biochemical characteristics of the microorganism. Fermentation of carbohydrates were used for CNS species differentiation, submitted to in vitro sensitivity test as of the in disc diffusion technique with 12 antimicrobial active principles: cefepime (30µg), ciprofloxacin (5μg), clindamycin (2µg), chloramphenicol (30µg), cotrimoxazole (25µg), erythromycin (15µg), gentamicin (10µg), oxacillin (1µg), penicillin (10IU), rifampin (5µg), tetracycline (30µg) and vancomycin (30µg). Sixty three CNS originated of 414 sheep were investigated, which S.xylosus (79.3%) being the most frequent, followed by S. saprophyticus (14.3%) and S. simulans (1.6%). Some species were not identified (4.8%). S. xylosus presented the biggest percentage of sensitivity to ciprofloxacin, gentamicin, cefepime and cotrimoxazole (100.0%, 98.0%, 96.0% and 96.0%, respectively) and the biggest percentage of resistance were penicillin, clindamycin, oxacillin and vancomycin (28.0%, 22.0%, 22.0% and 20.0%, respectively). S. saprophyticus and S. simulans were sensitive to all active principles tested. The species not identified were resistant (33.3%) to the same antimicrobial S. xylosus. Despite of the reduced percentage of resistance to the active principles tested, the realization of the in vitro sensitivity test for the selection of a suitable antimicrobial to the mastitis treatment may prevent the appearance of multiresistant strains and facilitate the disease healing.  Keywords: milk, microorganism, resistance.   Development agency: CNpq and FAPESP (Procedural 2013 / 23054-9)   

sensitivity to antimicrobial agents in different species of coagulase-negative staphylococci isolated in sheep's milk.

http://www.alice.cnptia.embrapa.br/bitstream/doc/1030041/1/ZAFALON2.pdf

sensitivity to antimicrobial agents in different species of coagulase-negative staphylococci isolated in sheep's milk.

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