GAVA, D.

KUCHIISHI, S. S.

LANGOHR, I. M.

RECH, R. R.

SCHAEFER, R.

SILVA, M. C.

ZANELLA, J. R. C.

Repository Open Access to Scientific Information from Embrapa

Abstract DetailSection: Diagnostic PathologyKeyword: Respiratory SystemPoster Number: Poster D-32PORCINE RESPIRATORY DISEASE COMPLEX INSOUTHERN BRAZIL: PATHOGENS AND ASSOCIATEDHISTOPATHOLOGYM.C. Silva1, R.R. Rech2, D. Gava2, S.S. Kuchiishi1, R. Schaefer2, I.M. Langohr3, J.R. Ciacci-Zanella2, 1CEDISA and 2Embrapa Swine and Poultry, Conc—rdia, SC, Brazil, 3College ofVeterinary Medicine, Michigan State University, Lansing, MI, USAEighty-six lungs from suckling (6%), nursing (65%), and fattening (29%) pigs with respiratorysigns from pig farms in southern Brazil were submitted to a diagnostic laboratory for necropsyand/or histopathological examination and screening of respiratory agents involved in the porcinerespiratory disease complex (PRDC). Lung samples were positive for influenza A virus (IAV)using real-time RT-PCR (RT-qPCR) (65%); the majority of the cases (95%) were also positivefor A(H1N1)pdm09 matrix gene using RT-qPCR, and 14% were positive for porcine circovirus-2 (PCV-2) using PCR. Forty-three samples (50%) had histopathological lesions associated withIAV characterized by necrotizing bronchiolitis/bronchitis and/or bronchointerstitial pneumoniawith bronchiolar/bronchial hyperplasia. In those cases, 58% (25/43) were positive byimmunohistochemistry (IHC) for IAV antigen. RT-qPCR testing was more sensitive than IHC indetecting IAV. Twenty-seven lung samples (31%) were positive for Mycoplasmahyopneumoniae (Mhyo) using IHC. Coinfection of IAV and Mhyo was seen in 17 (20%) cases.The hallmark lesion of Mhyo infection was peribronchial and peribronchiolar lymphoidhyperplasia. The most common isolated bacteria were Pasteurella multocida type A (8/18),followed by Haemophilus parasuis (4/18), Actinobacillus pleuropneumoniae (3/18), andSalmonella Choleraesuis (3/18). Most bacterial infections were associated with suppurativebronchopneumonia and/or pleuritis. Interstitial pneumonia was the typical lesion observed withSalmonella Choleraesuis and PCV-2 infections. All samples were negative for porcinereproductive and respiratory syndrome virus (PRRSV) using RT-qPCR. Influenza A virus andMhyo infections were the most common agents found in PRDC in pigs in southern Brazil.Furthermore, the screening for PRRSV did not detect this agent, consistent with the PRRSV-freestatus of Brazil. ©2013 AMERICAN COLLEGE OF VETERINARY PATHOLOGISTS

Porcine respiratory disease complex in southern Brazil: pathogens and associated histopathology.

Abstract

Similar works

Full text

Available Versions

Repository Open Access to Scientific Information from Embrapa