CASSOL, P. C.

COSTA, M. D.

GRAVE, R. A.

HIGARASHI, M. M.

MEZZARI, M. P.

NICOLOSO, R. da S.

SILVA, M. L. B. da

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© Copyright 2014 - Copyright Information, Privacy Statement, and Terms of UseAmerican Society of Agronomy | Crop Science Society of America | Soil Science Society of America5585 Guilford Road | Madison, WI 53711-5801 | 608-273-8080 | Fax 608-273-2021Certification 608-273-8085 | Fax 608-273-2081StartBrowse bySection/Division ofInterestAuthor Index145-6  Correlating Denitrifying Catabolic Genes with Soil N2O Emissions UnderContrasting Soil Disruption and Organic Amendments.See more from this Division: SSSA Division: Soil Biology & BiochemistrySee more from this Session: Soil Biology & Biochemistry: IMonday, November 3, 2014: 10:35 AMLong Beach Convention Center, Room 103BShare |Roberto André Grave1, Rodrigo da Silveira Nicoloso2, Marcio Luis Busi da Silva2, Melissa Paola Mezzari3, Martha MayumiHigarashi2, Paulo Cezar Cassol4 and Morgana Dalla Costa5, (1)Instituto Federal Catarinense, Concordia-SC, Brazil(2)Embrapa Swine and Poultry, Concordia-SC, Brazil(3)UNOESC, Videira-SC, Brazil(4)UDESC, Lages-SC, Brazil(5)FACC, Concordia-SC, BrazilAgriculture accounts for up to 84% of the anthropogenic N2O emissions to the atmosphere. Soil tillage and fertilization practices canaffect soil abiotic factors (e.g., pH, temperature, water saturation, nitrate and labile organic carbon contents) and consequently theabundance of nitrifying and denitrifying bacteria communities that regulates N2O efflux from soils. Nonetheless, the impact of organicN sources on bacterial communities and N2O production in aerated soils under conventional tillage and no-till systems remainsunclear. Labile C-rich organic fertilizers [i.e. raw swine slurry (RS)] could stimulate oxygen consumption by the growth ofheterotrophic bacteria respiration leading to anoxic environment that are not conducive for complete denitrification. Contrastingly, theapplication of anaerobically digested (ADS) or composted (CS) swine slurry could offset soil N2O emission by limiting substrateavailability for both nitrifying and denitrifying bacteria. We evaluated short-term N dynamics and N2O emission from a RhodicKandiudox under contrasting soil disruption [undisturbed (US) and disturbed soil (DS)] and N sources (140 kg N ha-1 as urea, RS,ADS, CS, and a control treatment without N). Soil abiotic factors were correlated with real-time quantitative PCR (qPCR) data toestimate concentrations of bacteria community harboring specific catabolic nitrifying-ammonium monooxygenase (amoA), anddenitrifying nitrate- (narG), nitrite- (nirS and nirG), nitric oxide- (norB) and nitrous oxide reductases (nosZ) genes. Water-filled porespace (WFPS) was consistently higher in the US and increased N2O emission in comparison to DS when WFPS was higher than0.6 cm3 cm-3. N2O emission from US amended with ADS and CS was 47.5 and 16.6% lower than RS (5.6 kg N2O-N ha-1),respectively. Correlations between soil abiotic factors and the distribution of nitrifying and denitrifying bacteria specific genes will bediscussed in attempt to demonstrate the effects of organic fertilization and their implications on N2O emission under contrasting soildisruption levels.See more from this Division: SSSA Division: Soil Biology & BiochemistrySee more from this Session: Soil Biology & Biochemistry: IPrevious Abstract | Next Abstract >>Abstract: Correlating Denitrifying Catabolic Genes with Soil N2O Emi... https://scisoc.confex.com/scisoc/2014am/webprogram/Paper85272.html1 de 1 25/11/2014 14:06

Correlating denitrifying catabolic genes with soil N2O emissions under contrasting soil disruption and organic amendments.

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