CUNHA, C. D.

LEITE, S. G. F.

MELO, I. S. de

RIZZO, A. C. L.

SORIANO, A. U.

TAKETANI, N.

TAKETANI, R. G.

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Thursday 28 AugustInfluence of nickel in petroleum biodegradation: a metagenomics approachNatalia Taketani*1, Rodrigo Gouvêa Taketani2, Itamar Soares de Melo2, Andréa Camardella de Lima Rizzo1, Adriana Ururahy Soriano3, Selma Gomes Ferreira Leite4, Cláudia Duarte da Cunha11CETEM /  MCTI, Brazií, 2cnpma/Embrapa, Brazií, 3PETROBRAS, Brazií, 4UFRJ /EQ, BraziíIndustry of energy is responsible for most of the soil multi-contamination with organic compounds and metais. The negatives effects of metais in the biodegradation of organic compounds turn the decontamination of these multi-contaminated sites challenging. Understanding the microbiological processes can help manipulate of remediation strategy. The aim of this study was to evaluate the effects of nickel on the biodegradation of crude oil in a tropical soil. To achieve this, we did a microcosm experiment under 4 conditions: soil (control), soil contaminated with oil (5% w/w), with oil (5% w/w) and nickel (260 mg/Kg) and soil contaminated with nickel during 30 days. Metagenomics and 16S rRNA libraries were sequenced using lon Torrent technology. The results showed that taxonomic changes were more affected by oil contamination and the order of Actinobacteria was predominant and increased in the presence of oil. The abundance of sequences related to organic compoundsdegradation increased in oil and Ni containing treatment, in contrast to previous studies that shown the negative effect of metais in degradation activity. Even nickel caused significant changes in the microbial community, it was possible to observe a functional redundancy between p-proteobacteria and Actinobacteria (oil, oil-Ni, respectively) compared the degradation of aromatics compounds, this kind of redundancy can explain the fact that the leveis of removal have not been different between treatments. This soil, regardless of the changes undergone by different pollutants, was able to remove the oil accessing microbial diversity, demonstrating the importance of the preservation of genetic resources in the environment.PS22 -  Biodegradation of challenging contaminants606

Influence of nickel in petroleum biodegradation: a metagenomics approach.

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