KMIT, M. C. P.

MENDES, R.

ROMAGNOLI, E. M.

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Hechos Microbiol. 2014; 5(2 Suppl 2).TLP-396. Metagenomic analysis of microbiome structure and functions in sheep rumenMaria Carolina Pezzo K.*, Emiliana Manesco R Rodrigo Mendes**LuizDe Queiroz College OfAgricutture (ESALQ), University OfSão Paulo, Piracicaba SP, Brazi! And Laboratory OfEnvironrnenta! Microbiology, Embrapa Environment, Jaguariúna SP, Brazil.Introduction. Plant biomass is a resource available at large scale with important application for second-generation biofuel production. The enzymes costs associated with their low efficiency represent a big ba- rrier for biofuel production economically viable from biomass. However, biomass degrading enzyme recovery from naturally enriched environ- ments, such as the rumen, offers a promising strategy for identification of new enzymes with higher lignocellulosic activity. Search for biomass degrading enzymes in the rumen of sheep fed with a diet amended with sugarcane bagasse in comparison with control treatment.Materials and methods. Sheep were fed for 60 days with a diet inclu- ding sugarcane bagasse or without bagasse (control) under experimen­tal conditions considering three replicates. Metagenomic DNA was ex- tracted from the solid contents of rumen followed by sequencing using miseq illumina plataform. The illumina sequence data was quality filte- red and assembled according to the mg-rast pipeline. The scaffolds were subject gene prediction using mg-rast and comparative analysis were performed using stamp.Results. The bacteria domain represented CA. 97% of reads conside­ring the total community. Bacteroidetes phylum was the most abundant(~48%), followed by firmicutes (~33%) and proteobacteria (6%). Pcoa analyses showed no significant difference between microbiome structu- res across treatments, nevertheless, samples from different treatments clustered when the functional profile was considered. Targeting specifíc hydrolase enzymes in the metagenomes revealed that beta-xylosidase, family gh43 (p=0.023) and diadenosine tetraphosphate (ap4a) hydrola- se (p=0.047) are significantly more abundant in the animais fed with a diet amended with sugarcane bagasse.Conclusions. Considering the sheep rumen as an untapped source of potential biomass degrading enzymes using a diet amended with sugar- cane bagasse is possible to increase the abundance of hydrolases genes.155

Metagenomic analysis of microbiome structure and functions in sheep rumen.

http://www.alice.cnptia.embrapa.br/alice/bitstream/doc/1010911/1/2014RA102.pdf

Metagenomic analysis of microbiome structure and functions in sheep rumen.

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