CHIARAMONTE, J. B.

FLORES, S. W. S.

MENDES, R.

ROMAGNOLI, E. M.

ROSSMAN, M.

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Presentaciones en Póster / Poster PresentationsTLP-218. Quantification of bacteria and archaea in rhizosphere of wild and modern common beanJosiane Barros C S t a l i n  Sarango F.*, Emiliana Manesco R Maike Rossman*, Rodrigo Mendes** Universidade Estadual de São Paulo, Brasil.Introduction. Rhizosphere microbiome has received ample attention in research considering that distinct crops harbor a rich and diverse rhi- zobacterial community. Recently was postulated that due to genetica- lly improvement plants may have lost their ability to recruit beneficiai bacteria. In this context, the comparison of wild and modern materiais is key to understand the selection of microbes in the rhizosphere by the host plant. In this study we aimed to quantify bacterial and archaeal 16s rRNA gene by using QPCR and compare their abundance in bulk soil and rhizosphere ofwild plants and modern cultivars ofcommon bean. Materials and methods. The experiment was performed using two cul­tivars, i.e midas and iac-alvorada and two wild plants, i.e. gl2873 and wild mex. Plants were grown in greenhouse conditions considering four replicates and sampled during the floweringstage. Total DNA was extrac- ted and bacteria and archaea 16s rRNA gene was quantified using QPCR. Results. In two independent experiments the amount of bacteria or archaea in the rhizosphere ranged from 2- to 8 fold when compared with bulk soil. No significative difference in abundance was observed when gl2873 (wild) and midas (cultivar) were compared. However, in the second experiment, which included wild mex and iac alvorada, the wild plant was able to harbor higher abundance of bacteria (p=0,0001) when compared with the modern cultivar iac alvorada. Interestingly, the abundance of archaea was higher (p=0,0019) in the midas rhizosphere when compared with the wild plant g12873.Conclusions. The plant is able to attract and enrich bacteria and archaea in the rhizosphere when compared with bulk soil. The results obtained from iac alvorada and wild mex experiment, suggests that ancestral ma­teriais communicated with microbes resulting in a higher quantity of bacteria in its rhizospHere than improved cultivars.110

Quantification of bacteria and archaea in rhizosphere of wild and modern common bean.

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