We started a project to explore American Oil Palm genetic resources through a combination of genetic and genomic approaches.Pôster W551

ALVES, A. A.

CAPDEVILLE, G. de

FORMIGHIERI, E. F.

LEAO, A. P.

LOBO, F. P.

RIOS, S. A.

SOUZA JUNIOR, M. T.

Repository Open Access to Scientific Information from Embrapa

When:January 10 - 15, 2014Where:San Diego, CAW551Exploring American Oil Palm (Elaeis oleifera) Genetic Resources in Brazil through a Combination of Genetic and Genomic ApproachesDate: Sunday, January 12, 2014Time: 3:50 PMRoom: TowneAlexandre Alonso Alves , Embrapa Agroenergy, Brasília, DF, BrazilEduardo Fernandes Formighieri , Embrapa Agroenergy, Brasília, BrazilAndré Pereira Leão , Embrapa Agroenergy, Brasília, BrazilFrancisco P. Lobo , Embrapa Informática Agropecuária, Campinas, SP, BrazilSara Almeida Rios , Embrapa Western Amazon, Manaus, BrazilGuy de Capdeville , Embrapa Agroenergy, Brasília, BrazilManoel Teixeira Souza Junior , Embrapa Agroenergy, Brasília, BrazilPDF fileThe Oil Palm expansion areas in Brazil coincide with the area of occurrence of bud rot, a major threat to the crop. Because of that breeders are nowusing E. oleifera germplasm in Oil Palm breeding programs, generating inter-specific hybrids. The species, however, lack the genomic resourcescurrently available for Oil Palm. Therefore, we started a project to explore American Oil Palm genetic resources through a combination of genetic andgenomic approaches. We’ve obtained so far, using the DArTSeq platform, over 7.000 silico-DArTs markers and 5.500 high-quality SNP loci, that werefirst used to evaluate the extent and organization of the genetic diversity of the collection of E. oleifera maintained by Embrapa. In parallel we startedthe whole-genome sequencing of the E. oleifera using a low cost hybrid-strategy. In phase 1 we sequenced and assembled (N50 ~2Kbp) three E.oleifera genotypes (each on 1 Illumina HiSeq 2000 lane). From phase II onwards, we concentrated our efforts in sequencing one genotype (fromManicoré, Brazil), using libraries with different insertion sizes in the Illumina platform (180 and 800bp PE and 3, 5 and 10Kbp MP libraries). The phase2 assembly showed a N50 of ~27Kbp, with >90% of filtered reads included into contigs. Despite the high coverage we obtained with the small reads(~100x), we decided to improve the scaffolding by sequencing large inserts (8 and 20Kbp) using the Roche 454 technology (Phase 3). We arecurrently working on a hybrid assembly strategy to use all the data we have in hands.Back to: Palm Genomics & Genetics<< Previous Abstract | Next AbstractHome/SearchBrowse by DayBrowse by TypeAuthor IndexPoster CategoriesMeeting InformationAbstract: Exploring American Oil Palm (<em>Elae... https://pag.confex.com/pag/xxii/webprogram/Pap...1 of 1 11-02-2015 09:43

Exploring American Oil Palm (Elaeis oleifera) genetic resources in Brazil through a combination of genetic and genomic approaches.

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