BOYD, L.

BRAMMER, S. P.

CASASSOLA, A.

CHAVES, M. S.

KUSER-FALCÃO, P. R.

NHANI JUNIOR, A.

STEFANATO, F.

ZERLOTINI, A.

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WHEAT TRANSCRIPTOME ANALYSIS TARGETING LEAF RUSTRESISTANCE-RELATED GENESA Casassola¹,  SP Brammer²,  MS Chaves²,  A  Nhani  Jr²,  JA Martinelli³,  PR  Kuser-Falcão4, A Zerlotini4, F Stefanato5, L Boyd51 Universidade de Passo Fundo, ² Embrapa Trigo, ³ Universidade Federal do Rio Grandedo Sul,4 Empresa Informática Agropecuária,5 National Institute of Agricultural BotanyLeaf rust is an important disease affecting wheat production  in Brazil, due toboth  production  losses  and  increased  management  costs.  Plant  pathogen  fungicideresistance demands  discovery  of  new  disease  management  control  strategies,  andimproved genetic breeding is one such effective approach. Toropi is a Brazilian wheatcultivar that shows an important characteristic for Brazilian wheat breeding; adult plantresistance (APR) to leaf rust. In order to better understand the leaf rust APR in Toropiwe compared RNA-Seq from Toropi, at different time points after inoculation (0, 6, 12,24 hai) with  Puccinia triticina, to the available Chinese Spring (CS42) transcriptome,identifying putative genes unique to Toropi. Reads from Toropi libraries were mappedto the CS42 transcriptome, as well as a bulk of the Toropi libraries being used to build areference  transcriptome  via  de  novo assembly.  Contigs  from  the  Toropi  de novoassembly  were  submitted  for  similarity  analysis  using  Blast  against  the  CS42trancriptome to identify contigs unique to Toropi. Differential expression (DE) analyseswere performed using RSEM and EdgeR. Around 58-59% of Toropi reads were mappedto the CS42 transcriptome, while around 66K contigs presented no homology. Amongthe  10,181  DE contigs,  9,156  were  present  in  both  Toropi  and  CS42,  while  1,025contigs which were differentially expressed in Toropi in the presence of the fungus hadno  hits  in  CS42.  These  Toropi-specific  sequences could  represent  new  resistancecandidate genes to be used in wheat breeding and need further study to determine theirrole in plant defense.Suported by: CAPES, CNPq, BBRSC and FAPERGS.

Wheat transcriptome analysis targeting leaf rust resistance-related genes.

http://www.alice.cnptia.embrapa.br/alice/bitstream/doc/1007511/1/WheatCasassola.pdf

Wheat transcriptome analysis targeting leaf rust resistance-related genes.

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