HANADA, R. E.

LOBO, I.

SILVA, A.

SILVA, G. F. D.

SOUSA, N.

SOUZA, A.

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CD ROM :: 46º Congresso Brasileiro de Fitopatologia Resumo:649-1649-1PCR-restriction fragment length polymorphism (PCR-RFLP) can be used for speciesidentification from Fusarium decemcellulare complex(PCR-RFLP permite a identificação de espécies do complex Fusarium decemcellulare) Autores: SILVA, G. F. D. (EMBRAPA - Embrapa Amazônia Ocidental) ; LOBO, I. (EMBRAPA -Embrapa Amazônia Ocidental) ; SOUZA, Á. (EMBRAPA - Embrapa Amazônia Ocidental) ;SILVA, A. (EMBRAPA - Embrapa Amazônia Ocidental) ; SOUZA, N. (EMBRAPA - EmbrapaAmazônia Ocidental) HANADA, R. E. - rhanada@inpa.gov.br (INPA - Instituto Nacional dePesquisas da Amazônia)ResumoFusarium decemcellulare  (teleomorph Albonectia rigidiuscula) is reported as the causal agent of diseaseswith varied symptoms in more than 50 plant species. In Brazil guarana plant can be highlighted as species ofeconomic  importance  affected  by  this  pathogen.  The  disease  in  guarana  plant  (Paullinia  cupana)  ischaracterized by floral hypertrophy, oversprouting and galls on the stalk and is currently one of the mainproblems in the field. The development of a molecular method for identification can be used to avoid anyconfusion  caused  by  morphological  identification.  Therefore  this  study  aimed  to  develop  a  method  formolecular  identification  by  PCR-RFLP  to  F.  decemcellulare.  For  the  method  development,  42  rDNAsequences of 24 species of Fusarium genus were aligned and restriction sites analyzed in silico. The methodwas  validated  using F.  decemcellulare  isolates  from guarana  plant  and  F.  solani  f.  sp.  piperis  and  F.oxysporum  f.  sp.  cubense as control. Amplification was performed using the  primer NL4 and ITS5 withsubsequent cleavage using HaeIII. The lack of the first restriction site located in the ITS 1 region was due tothe insertion of five nucleotides (GCTCG) between two cytosine generating a fragment of ~500bp in both A.rigidiuscula and A. albosuccinea and smaller fragments in other species of Fusarium that have at least 5restriction  sites.  The  results  confirm  the  efficiency  of  the  method  developed  here  to  identify  F.decemcellulare by restriction polymorphism.Apoio: CNPqCD ROM :: 46º Congresso Brasileiro de Fitopatologia file:///E|/resumos/R0649-1.html1 de 1 29/10/2013 15:01

PCR-restriction fragment length polymorphism (PCR-RFLP) can be used for species identification from Fusarium decemcellulare complex.

http://www.alice.cnptia.embrapa.br/alice/bitstream/doc/969742/1/Fusarium46CongressoBrasileirodeFitopatologia.pdf

PCR-restriction fragment length polymorphism (PCR-RFLP) can be used for species identification from Fusarium decemcellulare complex.

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