Four microsatellite sequences were tested in soybean DNA from cultivars and segregating genotypes. Three of them were close to the resistance locus rhg 1 on molecular linkage group G (Satt 309, Sat_168, Sat_163) and one was close to Rhg4 locus on group A2 (Sat_162). Progenies previously classified as cyst nematode (SNC) resistant and others with unknown reaction were tested, using the resistant cultivars Liderança, and Renascença and the susceptibles 'Cristalina' and 'OCEPAR-4' as control. The best primer for resistance to SCN was Sat_162. Twenty segregant progenies tested with Sat_162 presented a 150 bp band for homozygous resistant genotypes and 200 bp for susceptible ones, and both for the heterozigous genotypes. Previous studies have shown that this microsatellite marker is efficient to select genotypes carrying Peking-derivative resistance. 'Peking' takes part in the in the genealogy of all segregant progenies evaluated in this study. Thus, the obtained results showed that Sat_162 distinguished resistant and susceptible soybean genotypes to SCN, race 3
