MELO, I. S. de

VALARINI, G.

VALARINI, M. J.

VALARINI, P. J.

MELO, I.S. de

VALARINI, P.J.

RCAAP - Repositório Científico de Acesso Aberto de Portugal

Leaf yeasts as bioindicators of air pollution in southeastern Brazil.

Repository Open Access to Scientific Information from Embrapa

Proceedings ofthe 1dIJ Intemational Conference on Environmental Science and Technology Kos island, Greece, 5 - 7 September 2007 LEAF YEASTS AS BIOINDICA TORS OF AIR POLLUTlON IN SOUTHEASTERN BRAZIL 'Guilherme VALARINI; 'Itamar S. MELO; 2M.J. VALARINI and 'Pedro J. VALARINI 'Laboratory of Environmenlal Microbiology. Embrapa, CP. 69, CEPo 13820-000 Jaguariúna, SP, Brazir 'Instilulo de Zootecnia, Nova Odessa, SP. e-mail: lIamar@cnpma.embrapa.br ABSTRACT Phylloplane yeasts are susceplible lo a wide variety of environmenlal fluctualions such as urban and industrial air pollulion. Surveys of air qualily using leaf yeasts as bioindicators have never been carried oul in BraziL By using lhe spore-fall melhod, leafyeasl populalions were surveyed from lhe phyllosphere of Bauhinia forficata, Tabebuia sp., and Termina/ia catappa, wide-spread Iree species in urban environmenls of Brazi!. Two cilies from São Paulo Slate, represenling high pollution levei (Cubalão) and air good quality (Águas de São Pedro), were leaf Iree sampled in winler and summer seasons of 2005 and 2006. The resuHs demonslraled lower counls of leafyeasl for urban area of Cubalão indicaling Ihal such yeasts are sensilives lo pollulanls of lhe air, specifically lo sulphur dioxide of which levei reached around 50 ug/m3 in lhe winler, concenlration of which lhe yeasls respondo Between Irees, Ihere was significanl difference (p<0.1) for ipê (Tabebuia sp.) and chapeu de sol (Terminalia catappa) ai lhe winler lerm for Águas de São Pedro. Air pollulion seems lo affecl leafyeast populalions for ali Irees in lhe same way. The most common yeasl isolaled was Sporob%myces roseus, a ballistosporous basidiomycele species. Olhe r ballistosporous yeasts, colonies of which were Iypically white, were infrequently isolaled by lhe used melhod « 5%). A small subsel of isolales exhibiting variation in colony morphology was idenlified as Aureobasidium pullu/ans, Pseudozyrna antarctica, Cryptococcus a/bidus, Candida gui/lermondií and Candida sp. The data may have validity for olher lropical areas where sludies should be addressed lo lhe praclical use of leafyeasls as bioindicators for moniloring lhe air qualily. 1.INTRODUCTION The planl leaf surface, lerrned phyllosphere, supporls lhe growlh of a diverse microbiola of bacteria and fungi Ihrough lhe ulilization of lhe resources available in Ihis habitai (1; 2; 3; 4; 5). Therefore, phylloplane microorganisms occupy prominenl posilions in planl canopies. Among of Ihis epiphytic phyllosphere communily, yeasts are found in a wide variely of Irees in lemperale and lropical regions (6; 7; 8). They exisl bolh as small colonies and single cells concenlraled on lhe junctions between epidermal cells of leaves. The colonies increase in size by budding, as do Irue yeasls, bul also actively discharge spores, mainly at night (6). Since lhe leaves of higher planls have evolved as gas-exchange organs, in Iheir relalively exposed posilion may act as effeclive pollulanl Iraps. Leafyeasts are, therefore, in a very exposed position conceming lo lhe physico-chemical effects of gaseous and parliculale pollulanls of lhe air such as ozone, heavy melai and sulphur dioxide (9; 10; 11). Dowding and Carvil! (12) were lhe firsl lo reporl lhe negative influence of urban almospheres on Sporob%myces spp. inhabiling leaves of ash planls growing in a series of urban areas of Ireland. Furlher studies revealed Ihal fungi and leafyeast populalions were parlicularly sensilive lo air pollutanls, mainly sulphur dioxide (6; 7; 13; 14). Nakase et aI. (8; 15), in lhe course of a survey of yeasts in lhe phyllosphere from planls in Taiwan, found yeasl slrains belonging lo lhe genus 8-863 Sporob%myces. However, no reports have been published on pattern of air qualily revealed by lhe impacl of pollulion over leafyeasl populalions or anolher relaled lo lhe counling of yeasls from lhe phyllosphere of Irees, in Brazil. Our purpose was isolale ballislosporous yeasls trom lhe phyllosphere of Bauhinia forficata (pala de vaca), Tabebuia sp. (ipê), and Termina/ia catappa (chapeu de sol), wide-spread Iree species in urban environmenls of Brazil. Two cilies from São Paulo Slale (soulheasl of Brazil) were chosen lo be sampled in winler and summer seasons of 2005/2006, one represenling high pollulion levei from induslrial aclivity (Cubalão) and lhe olher, represenled high quality of lhe air (Águas de São Pedro). 2. MATERIALS ANO METHOOS Study sites: Leaf samples were collected from Bauhinia forficata (pala de vaca), Tabebuia sp. (ipê), and Terminalia catappa (chapeu de sol), grown in urban area of Águas de São Pedro (22°35' S, 47°52' W) ai 470 m above sea levei and Cubalão ai sea levei, cilies of São Paulo Slale, soulheasl of Brazil. The climale of bolh cilies is warm-humid ai summer wilh scarce precipilalion during lhe winter monlhs. Mean annual precipilalions are aboul 500 mm and 2541 mm lo Águas de São Pedro and Cubalão, respeclively. AI lhe leaf sampling, lhe lemperalure/air humidity were 23°C/56% and 22°C/66% for winler season and 28°C/62% and 24°C/81% for summer season lo Águas de São Pedro and Cubalão, respeclively. The mean concenlralions, over 2005 year, of lhe mosl air pollulanls, in Ilg/m3 , from Cubalão sampled siles were: SO" 39; NO" 89; 0 3 , 87 and inhalanl particulale >10 (PM10), 89. These data were extracted from lhe CETESB database (16), lhe air qualily managemenl bureau for São Paulo Slale. Lea' sampling: Four individuais of Bauhinia forficata, five of Tabebuia sp., and eighleen of Terminalia catappa, grown in urban area of Águas de São Pedro and Cubalão, were lagged and mapped using GPS syslem. In each Iree, Ihree green leaves from N,S,E,W directions were picked on a random basis at July 2005 (winter sampling) and January 2006 (summer sampling). The leaves were Iransferred, under cooling, lo lhe laboralory lo survey phylloplane yeasls. For isolaling lhe ballislosporous phylloplane yeasls, il was used lhe spore-fall melhod according lo Dowding and Peacock (7). Thus, six 1-cm dia meter discs were cul around lhe rib of leaves of each of lhe sampled Irees. Using a lemplale, Ihese discs were sluck by Iheir adaxial (upper) surface lo Pelri dish lids, using a blob of pelroleum jelly. The lids were replaced on lhe petri dishes conlaining 2.5% ma~ agar. The plates were incubated, lid-uppermosl, at room lemperalure and afler 24h lhe plates were inverted and incubaled for a further 72h. Afler lhe incubalion lime, lhe number of cOlonies, pink colour in Iheir majorily, under each leaf disc were counled, recorded and idenlified according lo melhod described below. Each differenl yeasl morpholype was purified and mainlained on YM slanls or liquid nilrogen for laler identificalion. The yeasls were characterized by slandard melhods (17). Idenlificalions followed lhe keys of Kurtzman & Fell (18). Dala were analyzed as splil plol randomized design, where plols represenled lhe Irees and splil plol were directions (N,S,E,W). Slalislical analysis was performed using appropriale model in PROC MIXED from Statistical Program SAS 8.02 (19). Leafyeast counl means were compared using Tukey-Kramer lesl (p<0.1). Since lhe counls of ballistosporous yeast are non-normally distribuled, dala were log (count+1) Iransformed. 3. RESUL TS ANO OISCUSSION 3.1 Leafyeast survey This survey was carried oul for urban zones of Águas de São Pedro and Cubalão, where maximum concenlrations of pollulants, over lhe year, in Ilg/m3 , were 50, 105, 80 and 120 6-864 for SO" NO" 0 3 and PM10, respectively. SO, and NO" the most yeast deleterious pollutants, showed increasing trend for mean monthly concentrations at the dry cold season (May to September). According to CETESB, the bureau on air quality management of São Paulo State, this seasonal pattem reflects the meteorological conditions that difficult the pollutant dispersion. Tables 1 and 2 summarize the analysis of leafyeast counts for winter and summer periods, respectively. Significant variation in counts from different sites was observed. According to the pooled analysis, counts are significantly higher (p<O.01) on host trees from Águas de São Pedro a~ both seasons. Among the tree species, there was significant difference (p<O.1) in the yeast counts only for Tabebuia sp. and Terminalia catappa at the winter term for the location of Águas de São Pedro. It was possible to discern some collect sites of Cubatão where leaves yielded no colonies; this cleary reflected the industrial activity (refinery, chemical industry and port) of the zone. Curiously, some of these sites, where the recorded leafyeast counts were so low, were located not so close to the industrial area. This demonstrated the influence of the wind direction on carrying and spreading air pollutants across the environment, changing the pattern and levei of pollution close to the source. Interference of cardinal points on sporing leafyeast enumeration between leaves derived trom the same tree was observed only for Cubatão, specially for northern si de of plant canopies showing that they are susceptible to environmental fluctuations of air pollutants. Thus, it would suggest to be unnecessary to record different sides of trees trom unpolluted areas as the balneary Águas de São Pedro. Concerning to the influence of the solar radiation, Newsham et ai. (2) demonstrated that SporoboJomyces roseus was one of the most sensitive yeast to u.v-B radiation. According to CETESB, the pollution levei, specially for sulphur dioxide for which leafyeasts are particularly sensitive, was higher in winter season. Unlike to temperate zone, an advantage in using leafyeast survey in tropical species is that they are throughout the year evergreen trees. Given that ballistosporous yeasts rely upon spore discharge for dispersai on leaf surfaces, these organisms show strategic exposure to environmental fluctuations, specially air pollutants. 3.2 Leafyeast identification The most common yeast isolated by the spore-fall method was Sporobo/omyces roseus, a ballistosporous basidiomycete. Other ballistosporous yeasts, colonies of which were typically white, were infrequently isolated by this method « 5%). A small subset of isolates exhibiting wide variation in colony morphology was identified as Aureobasidium pullulans, Pseudozyma antarctica, Cryptococcus albidus, Candida guiJIermondií and Candida sp. 6-865 Tree Table 1: Pooled analysis of count means of leafyeast colonies isolated from the trees Bauhinia forficata, Tabebuia sp. and Tenninalia catappa collected at urban zones of Á ~Quas de São Pedro and Cubatão (SP) at lhe winler period. Águas de São Pedro Cubalão n n n Mean CS 28 2.17 ±0.15(1 ) ab (183 ±15)12) 44 1.35 ±0.12a (42 ±12) 72 '1.76 ±0.09a (112 ±10) IP 4 2.44 ±0.39a (313 ±40) 16 1.53 ±0.19a (62 ±20) 20 1.99 ±0.22a (187 ±22) PV 8 2.05 ±0.28b (138 ±28) 8 1.10 ±0.28a (12 ±28) 16 1.57 ±0.19a (75 ±20) N 10 2.19 ±0.20a (170 ±34) 17 1.54 ±0.14a (75 ±24) 27 1.86 ±0.12a (123 ±21) S 10 17 27 Direclion 2.33 ±0.20a (285 ±34) 1.44 ±0.14ab (39 ±24) 1.88 ±0.12a (162 ±21) Mean E 10 2.32 ±0.20a (232 ±34) 17 1.23 ±0.14bc (27 ±24) 27 1.78 ±0.12ab (129 ±21) W 10 2.06 ±0.20a (158 ±34) 17 1.09 ±0.14c (15 ±24) 27 1.58 ±0.12b (86 ±21) 2.22 ±0.17A (211 ±17) 1.32 ±0.128 (39 ±12) I') .IL), Mean transformed In log (count+1), Onglnal mean, n, observatlon number. Values followed by a different small lelter are significant by Tukey-Kramer test (p<O.1) and values followed by a different capilallelter are significant by F test (p<O.01). CS, Terminalia catappa; IP, Tabebuia sp.; PV, Bauhinia fot1icata. Table 2: Pooled analysis of count means of leafyeast colonies isolated from the trees Bauhinia forficata, Tabebuia sp. and Tenninalia catappa collected ai urban zones of Águas de São Pedro and Cubatão (SP) at the summer period. Águas de São Pedro Cubatão n n n Mean CS 28 2.18 ±0.14(1 ) a (182 ±11)12) 44 1.42±0.11a (41 ±9) 72 1.80 ±0.09a (111 ±7) Tree IP 4 2.40 ±0.37a (260 ±29) 16 1.57 ±0.19a (43 ±14) 20 1.99 ±0.21a (151 ±16) PV 8 2.08 ±O.26a (136 ±20) 8 1.19 ±O.26a (15 ±20) 16 1.64±0.19a (76 ±14) N 10 2.11 ±0.18a (156 ±25) 17 1.46 ±0.13a (35 ±18) 27 1.79±0.11a (96 ±15) S 10 2.22 ±0.18a (185 ±25) Direclion 17 1.26 ±0.13b (22 ±18) 27 1.74 ±0.11a (104 ±15) E 10 2.33 ±0.18a (227 ±25) 17 1.38 ±0.13ab (31 ±18) 27 1.86 ±0.11a (129 ±15) W 10 2.23 ±0.18a (202 ±25) 17 1.48 ±0.13a (43 ±18) 27 1.85 ±0.11a (123 ±15) Mean 2.22 ±0.16A (193 ±12) 1.40 ±0.118 (33 ±9) \')1 + . I"~, Mean transformed In log (count 1), Onglnal mean, n, observatlon number. Values followed by a different small lelter are significant by Tukey-Kramer test (p<O.1) and values followed by a different capital lelter are significant by F test (p<O.01). CS, Terminalia catappa; IP, Tabebuia sp.; PV, Bauhinia fot1icata. 8-866 REFERENCES 1. JACQUES, M. -A; MORRIS, E. M. 1995. A review of issues relaled lo lhe quanlificalion of bacleria from lhe phyllosphere. FEMS Microbiology Ecology 18,1-14. 2. NEWSHAM, K. K.; LOW, M. N. R; MCLEOD, A R; GREENSLADE, P D.; EMMETT, B. A 1997. Ullraviolel-B radiation influences lhe abundance and dislribulion of phylloplane fungi on pedunculale oak (Quercus robur). New Phytologisl 136, 287-297. 3. MERCIER J.; L1NDOW, S. E 2000. Role of leaf surface sugars in colonizalion of plants by baclerial epiphyles. Applied and Environmenlal Microbiology 66, 369-374. 4. JACOBS, JL; SUNDIN, G.W 2001. Effect of solar UV-B radialion OA a phyllosphere bacterial community. Applied and Environmenlal Microbiology. v.67, 5488-5496. 5. STOHR, S. N.; DIGHTON, J. 2004. Effects of species diversily on eslablishmenl and coexislence: a phylloplane fungai communijy model syslem. Microbial Ecology 48, 431-438. 6. DOWDING, P.; RICHARDSON, D.H.S. 1990. Leaf yeasl as indicalors of air qualily in Europe. Environmenlal Pollulion 66, 223-235. 7. DOWDING, P.; PEACOCK, J. 1991. The use of leaf surface inhabiling yeasl as monilors of air pollulion by sulphur dioxide. In: Bioindicators and environmental management, Jeffrey, D.W; Madden, B. (eds.) pp. 322-342, Academic Press Limiled, London, U.K. 8. NAKASE, T.; LEE, F-L.; AM-IN, S; JINDAMORAKOT, S.; POTACHARAROEN, W; TANTICHAROEN, M., TAKASHIMA M. J. 2005. Sporobolomyces fushanensis sp. nov., a new species of ballisloconidium-forming yeasl in lhe Microbotryum lineage isolaled from a planl in Taiwan. Journal General Applied Microbiology, 51: 41-45. 9. MAGAN, N.; MCLEOD, A R 1991. Effect of open-air fumigalion wilh sulphur dioxide on lhe occurrence of phylloplane fungi on winler barley. Agricullure, Ecosyslems and Environmenl 33,245-261. 10. MAGAN, N.; KIRKWOOD, I. A; MCLEOD, A R; SMITH, M.K. 1995. Effect of open-air fumigation wrth sulphur dioxide and ozone on phyllosphere and endophylic fungi of conifer needles. Planl, Cell & Environmenl 18, 291-302. 11. VASSILEVA E.; VELEV, V.; DAIEV, C.; STOICHEV, T.; MARTIN, M.; ROBEIN, D.; HAERDI, W. 2000. Assessmenl of heavy melais air pollution in urban and induslrial environments using oak leaves as bioindicalors. Inlernalional Journal of Environmenlal Analytical Chemislry 78, 159-173. 12. DOWDING, P.; CARVILL, P. H. (1980). A reduclion of counts of Sporobolomyces roseus Kluver on ash (Fraxinus excelsior L.) leaves in Dublin Cily. Irish Journal of Environmental Science.1: 65-68. 13. FELLNER, R; PESKOvA V. 1995. Effects of induslrial pollutanls on ectomycorrhizal relalionships in lemperale foresls. Canadian Journal of Bolany 73 (Suppl. 1), S1310-S1315. 14. TERZIYSKA, A; WALTSCHEWA, L.; VENKOV, P. 2000. A new sensilive lesl based on yeasl cells for sludying environmenlal pollulion. Environmenlal Pollulion 109, 43-52. 15. NAKASE, T. S.; LEE, F. L.; SUGITA, T.; JINDAMORAKOT, S.; JAN-NGAM, H.; POTACHAROEN, W; TANTICHAROEN, M.; KUDO, T.; TAKASHIMA, M. 2003. Sporobolomyces magnisporus sp. nov., a new yeasl species in lhe Erythrobasidium clusler isolaled from plants in Taiwan. Journal General Applied Microbiology 49, 337-344. 16. CETESB, 2005/2006. Companhia de Tecnologia de Saneamenlo Ambienlal. Relalório Mensal de Qualidade do Ar no Eslado de São Paulo, Série Relalórios, CETESB, São Paulo. Available in www.cetesb.sp.gov.br 17. YARROW, D. 1998. Melhods for lhe isolation, maintenance and identificanon of yeasts. In: Kurtzman C. P. and FeH J. W. (eds), The Yeast - A Taxonomic study. Elsevier, Science Publishers, Amsterdam, The Netherlands, p. 66. 18. KURTZMAN, C. P.; FELL, J. W The Yeasls - A laxonomic sludy. 4th ed. Elsevier Science Publishers, Amslerdam, 1998. 19. SAS INSTITUTE, 2004. SAS/STAT Software: changes and enhancemenls lhrough release 8.2. Cary: Slatislical Syslem Inslilule. 1028p. 8-867 

Leaf yeasts as bioindicators of air pollution in southeastern Brazil.

Abstract

Similar works

Full text

Available Versions

RCAAP - Repositório Científico de Acesso Aberto de Portugal

Repository Open Access to Scientific Information from Embrapa