ARRUDA, L. F.

BORGHESI, R.

DAL'BÓ, G. A.

GONÇALVES, V. T.

HISANO, H.

LOSEKANN, M. E.

OETTERER, M.

SAMPAIO, F. G.

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5° Sim1pósi,o Inte~naci'Qnalde Nutriçã.o eS'aúde de :PeixesFMZ - UNESp· - Botucaw, s· a 8 d@novembro de 201JHematological changes in pacu (Piaractus mesopotamicus) in response to differentlevels of fish silage as fish feedVitória Teodoro Gonçalves*l, Fernanda Garcia Sarnpaioã, Marcos Eliseu Losekann2,Hamilton Hisanoê, Lia Ferraz de Arruda4, Ricardo Borghesi5, Marília Oetterer6,Genoefa Amália DaI 'Bó 7*lGraduanda em Engenharia Ambiental; Faculdade de Jaguariúna, Jaguariúna - SP; 2Pesquisadores,Empresa Brasileira de Pesquisa Agropecuária - Embrapa Meio Ambiente; Rod SP 340, km 127,5 -13820-000 - Jaguariúna - SP - femanda.sampaio@embrapa.br; 3Pesquisador, Empresa Brasileira de PesquisaAgropecuária - Embrapa Agropecuária Oeste, Dourados, MS; 4 Doutora em Ciências, Centro de EnergiaNuclear na Agricultura da Universidade de São Paulo (CENA - USP), Piracicaba, SP, Brasil;5Pesquisador, Empresa Brasileira de Pesquisa Agropecuária - Embrapa Pantanal, Corumbá, MS; 6Professor do Departamento de Agroindústria, Alimentos e Nutrição da USP, Piracicaba, SP, Brasil;7Pós Graduanda em Patologia Clínica Veterinária; Instituto Brasileiro de Veterinária.The use of fish silage in fish feed is an important alternative to utilize fish waste withpotential source of minerals, fatty acids and high quality protein in aquaculture diets.This study investigated the effect of levels of fish silage in experimental diets on theerythrogram, leukogram and hematimetrics index of the pacu, during 60 days. 120juveniles ofpacu (Piaractus mesopotamicus) were randomly distributed in 15experimental glass aquaria, in a closed recirculation system, with constant temperatureand aeration. Fish were divided into 5 experimental groups: 0% control (GO), 25%(G25), 50% (G50), 75% (G75) and 100% (GlOO) offish silage, 3 aquariapertreatment,and fed twice daily to apparent satiation. The water quality parameters in the aquariumwere daily measured. At the end of the experimental period, after 24 hours fasting, fishwere anesthetized with benzocaine and blood was collected from caudal puncture withheparinized syringe, for hematological analysis. Data were undergoing analysis ofvariance (ANOVA), with application ofTukey's test (P<0.05) trough SAS software. Nomortality was recorded in different experimental groups. No statistical differences wereobserved between the experimental groups. However, it was possible to observe thatGIOOpresented the lowest means ofRed Blood Cells (RBC), hematocrit (Ht) andhemoglobin (Hb) in relation to the other groups, showing a possible influence of higherlevei of silage inclusion in erythrogram. The group control GOpresented the highestmeans ofHt, Hb and Mean Corpuscular Hemoglobin Concentration (MCHC) in relationto the group G100. The group G50 presented the higher value of Mean CorpuscularVolume (MCV) when compared to the other treatments, while the group G25 presentedthe same means ofHt and MCHC found in the groups GOand 075, respectively. ln thepresent study all the experimental groups presented White Blood Cell (WBC) in asimilar range. ln relation to the variety of leukocytes the groups 00 and 050, presentedneutrophils, lymphocytes, monocytes and Special Granulocitic Cell (SOC). Curiously,025 was the unique group that did not present monocyte but at the same time presentedthe lowest level ofbasophils and SOC. The present study demonstrates that fish silagecan be used as an alternative protein source for pacu with no significant influence inhematological parameters.Support: CAPES (PNPD-0096083).1

Hematological changes in pacu (Piaractus mesopotamicus) in response to different levels of fish silage as fish feed.

Hematological changes in pacu (Piaractus mesopotamicus) in response to different levels of fish silage as fish feed.

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