Trabalho apresentado no World Congress on In Vitro Biology, 2000, San Diego, California

COELHO, M. C. F.

LAMEIRA, O. A.

PINTO, J. E. B. P.

SANTIAGO, E. J. A.

SILVA, F. G.

RCAAP - Repositório Científico de Acesso Aberto de Portugal

Seed germination and in vitro propagation of sucupira branca (Pterodon pubescens (Benth.) Benth.), a medicinal plant.

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VOLUME 36 NUMBER 3, PART II MARCH 20002000World Congress on In Vitro BiologyJune 10 - 15,2000San Diego, CaliforniaThe Town & Country ResortJournaloftheSociety forIn VitroBiology•i Plant - Poster Sessions-~º_<ilSeed Germination and In vitro Propagation of Sucupira Branca [Pterodonpubescens (Benth.) Benth.], a Medicinal Plant. J.E.B.P.PINTO;M.C.F.Coelho; O.A.Lameira; EJ.A.Santiago and F.G.Silva. LaboratoryTissue Culture:-'OFLA CX.P.37, Lavras, MG, 37200-000. email:jeduardo@ufta.brPterodon pubescens (Bcnth.) Benth. is a essence native to Brazilian cer-rados, reaching up to 16 meters in height. The oil of fruit is very enjoyedin folk medicine in sore and rheumatic infections, it protects from cercariainfections. The wood posses high natural resistence to rotting, being re-garded as one of the most resistent woods for railroad sleepers. The ob-jectives of the present work were to identify the best in vitro and ex vitrogermination conditions for the seed, embryo and embryonic axes of theseedlings development, in addition to determinating a methodology forin vitro multiplication. Experiments were conducted aiming at theachievement of in vitro germination by means of the use of modified MSbasic medium, in liquid medi um, different gelifyings, caps and light con-ditions the establishment of nodal segments were studied by employingthe MS and WPM basic medi um. In the multi-sprouting experiment, fourdifferent combincd concentrations of ANA and BAP were tested. Thebest type of cap for embryo germination was the one of aluminum papermould together with liquid mcdium the nodal scgments were best estab-lished in WPM medium. Up to 7,5 shoots/segment were obtained, withthe nodal segment, inoculated at the horizontal position in the MS culturemedium with half the concentration of salts, supplemented with 0,5 mMBAP.P-2064Multiplication Strategies for Hvpericum [oliosum Aiton, an Endemic Azorean Spe-cies. Graciete Belo Maciel and MONICA MOURA. Departamento de Biologia,Universidade dos Açores. Apartado 1422,9501-801 Ponta Delgada (Açores) Co-dcx, Portugal. Emai!: maciel@notes.uac.pt.moura@notes.uac.ptHvpericum [oliosum Aiton, is a beautiful Azorean endemic species whose popu-lations currently yield a low number of individuais in many af the archipelagoislands. In order to find an effective ex situ multiplication strategy for this plantseveral studies were carried out. During a 3 years span the germination capacity ofits seeds was tested. The essays took place under a cantinuous temperature of 15'C and 10-20 'C alternare, for a period 01' 8 hours light and total darkness. Inalternate temperature tests the light period was rnade to coincide with the highesttemperature. The species showed a positive phatosensitivity and gerrninatian per-centage of 67'7< under a continuous 15°C temperature. A short dorrnancy wasdetected in seeds with 3 months of conscrvation. At the end of 15 and 25 rnonths01' storage new essays were done and the results obtained showed a progressivereductian in the plants germination capacity. The first rnicropropagation studiescarried out revcalcd that the bcst mcdium to cultivate HvpcricumIoliosum'< singlenade cuttings was Côrte & Mendonça (1985), supplemented with 0.4 p.M N6-benzyladenine (BA) and 2.6 u.M a-naphthaleneacetic acid (NAA) + 4.4 u.M BA.in the initiation stage and OA u.M BA, in the elongation stage. Regarding culturemultiplication, 0.4 u.M BA. in the initiation stage and 2.6 u.M NAA + 4.4 u.MBA, in the initiation anel elongation stages, proved to bc the rnost efficient concen-trations. The acclimatization stage was also successfully performed in Jiffy 7'"pellets. To fine tune the composition 01' thc culture medium, several tests were thenrun using different quantities 01' sucrosc. Namely, 5, 10, 20, 30 and 40 g/l. A higherdiffcrentiation and multiplication rate was achieved in a 20 g/l concentratian. whichalso produced longer shoots anel the highest percentage 01' rooting. The pH intluencein Hvpericum culturcs ' performance was also tested using 3 different values: 4.8.5.8 and 6.8. Beuer differentiation and elongation valucs werc achieved in pH=5.8.as also was the percentage of rooted explants. Regarding multiplication, 5.8 alsoproduccd the best results.P-2065Chemically Induced Resistance 01' Carica Papaya against PhytophthoraPalmivora. Y. JUDY ZHU', Maureen Fitch-, Stephen Ferreira' and PaulMoore-. 'Hawaii Agriculture Research Center, Aiea, HI 98701, 'USDA,ARS, Aiea, HI 96701, lUniversity of Hawaii, Honolulu, HI 96822.E-mail: jzhu@harc-hspa.comAcquired resistance is an inducible defensc mechanism exhibited bymany plants that provides protection against a broad range of pathogcns.Systemic acquired resistance (SAR) has becn induced by treatment withchcmical substances such as salicylic acid (SA) and ben-zo(l,2,3)thiodizaole-7-carbothioic acid S-mcthyl ester (BTH) in both di-cotyledenous and moncotyledonous plants. Wc are cxploring the possi-bility of using BTH-induced SAR as an alternative approach for controlof the root rot and fruit rot diseases caused by Phytophthora palmivora.Here we report that in tropical fruit papaya, Carica papaya, chemicaltreatments with SA and BTH can induce SAR against Phytophthora pal-mivora. Young papaya plants pretreated with 15 mM SA showed 35-50%fewer lesions and smaller infected are as two weeks after inoculation withP. palmivora than similar plants without SA treatment. BTH increasedpapaya plant resistance to P. palmivora at concentration as low as 1.0mM. BTH at this concentration exhibited slight toxicity to the papayaseedlings but gave complete protcction against P. palmivora whereas con-trol plants treated with water showed over 70% mortality 5 days afterinoculation. Studies on the effectiveness 01' a range of concentrations ofBTH will be reported. Enzyme activities of the pathogencsis-related pro-teins chitinase and beta-1,3-glucanase increased more than six-fold fol-lowing BTH treatment indicating that BTH is acting as a chemical in-ducer of SAR in papaya.P-2066One-step, in vitro Acclimatization of Carnation using a Mist Reactor. MJ.CORRELL and P.J. Weathers. Dcpt. of Biology/Biotechnology, WorcesterPolytechnic Institute, Worcester, MA 01609. E-mail: mcorrell@wpi.eduMist reactors offer a variety of benefits when compared to conventionalmicropropagation techniques: the gas phase surrounding the plant tissuecan be readily manipulated, large quantities of plants can be culturcd ina single vessel, the liquid médium can bc regulated throughout plantdevclopment, and they can be easily automated. An acoustic window mistreactor was used for in vitro culturing and subsequent acclimatization ofDianthus caryophvllus L. Plant nodes were culturcd for fivc weeks ineithcr the mist reactor or GA7 culture boxes (Magema'>'), containing orlack.ing a 0.2 <FONT FACE=Symbol>m</FONT>m filter-vent in thelido For plants grown in the reactor, the medium fccd rate or misting cycleincreased over the culturing period from 2 to 4 to 10 minutes of mistingon per hour, for weeks I. 2, and 3-5, respcctively. In addition, the lastweek of in vitro culture within the reactor included a stepwise reductionin relative humidity from 99% to 70% Rh using dricd, ambient air toftush the headspace surrounding plant tissues. Plants from both the reactorand corrcsponding GA 7 boxes were transferred to the greenhouse for fiveweeks without additional acc1imatization. Plant survival was highest forplants grown in the mist reactor (89% survival) compared to plants grownin either GA 7 boxes (50% survival) or GA 7 boxes with filter-vents (81 %survival). Survival corrclated with low levels of hyperhydration. Theseresults show that by careful manipulation 01' the environment, one-stcpacclimatization can be achievcd using a mist biorcactor.:COllgress 011 I" Vitro Biofogy 71-A Abstracts

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Seed germination and in vitro propagation of sucupira branca (Pterodon pubescens (Benth.) Benth.), a medicinal plant.

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