ALBUQUERQUE, P. E. P. de

ANDRADE, C. de L. T. de

BASTOS, E. A.

GOMIDE, R. L.

GUIMARAES, C. M.

MORGADO, L. B.

RIBEIRO JUNIOR, W. Q.

VIANA, J. H. M.

Repository Open Access to Scientific Information from Embrapa

GCP Annual Research Meeting 2007Template for posterControlling and Monitoring Water Stress in Contrasting Environrnent for DroughtTolerance Phenotyping of Cereais and LegumesReina/do Lúcio Gomide-Embrapa Maize and Sorghum,Paulo Emí/io Pereira de Albuquerque,Cami/o de Le/is Teixeira de Andrade.Cleber Morais Guimarães,Edson AIves Bastos.Walter Quadros Ribeiro Júnior,Luiz Balbino Morgado,João Herbert Moreira VianaTheme: Exploiting Allelic DiversityAbstractDrought is considered the main source of grain yield instability for cereais and legumes intropical regions among other environmental stresses. The drought process studies requireknowledge of some target environment factors and how these factors interact with plantsgenotypes performance under water constraint conJition. The objectives of this work are todescribe the procedures and practices of controlling and monitoring water stress in contrastingenvironment for drought tolerance phenotyping of cereais and legumes to better understandthe effects of plants genetic and environmental (GxE) interactions for grain yield, identifyingand characterizing the causes which will result in genotypes yield reduction due to watershortage. Irrigation water needs are being determined through computation ofreference (ETo)and crop (ETc) evapotranspiration, using modified Penman-Monteith equation and cropcoefficient (kc). Each genotype ETc is being detcrmined through multiplying ETo by Kc.Irrigation management strategy and timing criteria are being performed based on aspreadsheet water balance program, including ETo, ETc, and soil water content at differentdepths. The water stress treatments are being obtained with different ETc replacement,generating different water depth applications in the plots at pre-defined crop growth phases,defined for each genotype, in order to establish the water stress level.lntroductionOrought is the main source of grain yield instability for cereais and legumes in tropicalregions among other environmental stresses. The drought process studies require knowledgeof SOme target environment factors and how thcse factors interact with plants genotypes. ,performance under water constraint condition. The objectives of this work are to describe theprocedures and practices of controlling and monitoring water stress in contrastingenvironment for drought tolerance phenotyping ot cereais and legumes to better understandthe effectsofplants genetic and environmental (Gx.E) interactions for grain yield, identifyingand characterizing the causes which will result in genotypes yield reduction due to watershortage.Irrigation Water ControlThe correct irrigation scheme design and layout 011 the site-specific experimental area areessential in lhe selection process of drought tolerant genotypes to make possible and easy theirrigation water control and management.12111r-: ------.j~---------"l.lUtlollllne valve•• •• ••• ••••• SoU• .•e. •e.-;-"m oistlll e~·..... ~2 m x2 mGlitl-; ••••• ..,~C*C'• •••••• •••••e e• •••••••• • ••e .e e e,Pressure tJilu4JeJoIotol-PumpFigure I. Conventional sprinkler irrigation system water control and measurements(sprinklers spacing, operating pressure, flow rate, application depth, uniform waterdistribution, and soil moisture).Crop Water Requiremcnts and Water StressCrop water needs (ET= Evapotranspiration) are being computed by means of modifiedPenman-Monteith equation and crop coefficient (kc) (ETo= reference, ETc= crop). Eachgenotype ETc is being calculated multiplying ETo by Kc. Irrigation management strategy andtiming cri teria are being performed based on a spreadsheet water balance program, includingETo, ETc, and soil water content at different depths. The water stress treatments are beingobtained with different ETc replacement, generating different water depth applications in theplots at pre-defined crop growth phases, defined for each genotype, in order to establish thewater stress level. The following crop water stress index (CWSI) is being used to quantify thewater stress (ETcact & ETcpot are ETc actual & potential):CWSI = 1_ E-Tcü<,"ETc paIThe crop water stress index results considered for analysis were taken from an irrigated beansfield under four different water regimes from the growth pcriod between the 29 and 80 daysafter seedling (DAS). All four water treatments received a total of 118.8 mm of applied waterfrorn seedling to 28 DAS, with 4-day average irrigation interval. Just after lhe 28 DAS,treatmcnt difTerentiation started, with the application 01' pre-determined depths 01' water,according 10 the irrigation intervals. From seedling to harvest, the total amounts 01' appliedwater, including 66 mm ofrainfall, were 439.6,3856,326.5, and 290.4 mm for T4, T8, T12and T16 treatments, respectively .W.••• fIow ~.~,.c_oI .1 ~...Figure 2. Environment characterization with monitoring & controlling sensorsl equipments/devices/ systems based on feedback measurements of the real time local micro-climatic condition, irrigation water application, and soil and plants water status.Treatrnerasx(j)"Oc: 0.10 '. ~ l\' . .:: ~!.\..• • ,..- ~, ...: • • I:"'· •• '\. ~•..• , ..•• \~.:. ,'. \ ./~I...' '.,"/ .- -,...'. ~ ~ \ ...0.00-t--------T--/tTI-+-~-+--o'--+--___'!~~------\i\,.0.25-.-. T<418T12, T160.20 ,.,0.15..'.,. "0.CX5-0.10-O.a550 60 70days after seedlingFigure 3. Crop water stress index for beans versus crop growth period (expressed as days afterseedling) at four irrigation frequencies (T.:1, T8, T 12, and T 16) for measurernents inthe rnorning period (EMBRAPA, Sete Lagoas, MG, Brazil, 2002)Figures 3 and.4 show the crop water stress index vanation with the crop growth period(expressed as days after seedling) at four irrigation írequencies (T4, T8, T12, and T16) andtwo measuring periods (moming and aftemoon), respectively. Treatment T4 was assumed tohave a crop with adequate water supply and maximum or potential evapotranspiration rate inthe CWSI determination. The results show that T4 presented less oscillation in the CWSlvalues which were maintained close to the zero leveI. Therefore, four-day irrigation frequencydid not cause water stress damage to the crop. BJ analyzing the two measuring periods(morning and afternoon), it can be noticed a similar tendency in the CWSI variation. But thehighest CWSI values were obtained during afternoon readings. At first, this suggests that thebest time for canopy temperature (Te) reading is between 13:00 and 14:00 hours for CWSIstudies.Treatmenl$0.4--e - T4T60.3T1~ã T T16x~ 0.2c:T.: .. .!lil!!. 0.11'FQjl b.oa.g..().1i i i30 40 50 60 70 ao 90days after seedlingFigure 4. Crop water stress index for beans versus crop growth period (expressed as days afterseedling) at four irrigation frequencies (T4, T8, T12, and T16) for measurements inlhe afternoon period (EMBRAP A, Sete Lagoas, MG, Brazil, 2002).The treannent that caused most water stress was T 16. where lhe CWSl reached va\ues 01' 0.26and 0.40· for -morning and afternoon periods, respectively. Overall, lhe results show that aCWSI value of about 0.15 may be used as a limit for irrigation water management strategiesto differentiate the irrigated crop from a non-stressed to a stressed condition, in order to avoidsignificant yield loss. This limit of 0.15 derives from the observation that T4 and T8treatments presented maximum CWSI values of roughly 0.10 and 0.20, respectively. At T4,results indicate that the crop was also adequately supplied with water (no-stress). On the otherhand, at T8 the crop may have experienced a certain degree of water stress.Consideration, Recommendation and Conclusions• Collecting environment data on water (irrigation & rainfall), climate condition, soils(moisture content), and cropping systems (phenology & physiology) is essential whenwater is in short supply (drought) and evaporative demand ofthe atmosphere is high inorder to better understand the effects of genotypes and environmental (GxE)interactions.• lrrigation water management involves three decisions i) How to apply the irrigationwater (Scherne), ii) When to irrigate (Tirning), and iii) How much water to apply(Quantity).• Wherever environment conditions are short in water supply and evaporative demandof the atmosphere is high, a localized scheme (micro-sprinkler or drip) is indicatedbecause the amount of water and the frequency of application can be controlled veryfinely, direct to the crops root zone.• The ETc = Kc x ETo computation is used to apply water in the whole area (ali thefield is wetted). For reduced wetted are a, when only part of the soil surface is wetted(drip), the ETc must be ca\culated different (ETc localized), using a reduction factorKr = GC + 0.5 (1 - GC)] and a ground cover (GC = fraction of the total surface areaactually covered by the crop foliage) byE'I'cloc= Kr . ETc = Kr . Kc . EToFor drought tolerance phenotyping of cereais and legumes studies, the CWSI valuesare in the range of 0.6 to 0.7 at the Ernbrapas contrasting environment.It was verified a similar tendency of the CWSI variation in lhe periods of measurement(morning and afternoon). The highest CWSl values v. cre obtaincd during afternoon readings,suggesting that as the best period for canopy temperature (Te) measurements. The highestwater stress was obtained with 16-day irrigation frequency, when the CWSI reached values of0.26 and 0.40, for moming and afternoon periods, respectively.A crop water stress index value of about 0.15 to 0.20 may be used as a limit for irrigationwater management to differentiate the irrigated crop from a non-stressed to a stressedcondition, as criteria to avoid significant yield loss.The infrared thermometry and energy balance/Bowen ratio techniques can be used todetermine crop water requirements, to detect crop water stress indexes, and to scheduleirrigation.References--------. 1973. Principies 01' environmental physics. Arner. Elsevier. N.Y 243 pp.Andrews, P.K., Chalmers, DJ. and Moremong, M. 1992. Canopy-air temperature differencesand soil water as predictors of water stress of apple trees grown in a humid,temperate climate. Journal American Society Horticultural Science, Mount Vernon,Vo1.II7, pp.453-8.Clawson, K.L., Jackson, R.D. and Pinter Jr., P.l. 1989. Evaluating plant water stress withcanopy temperature differences. Agronomy Journal., Madison, Vo1.81, pp. 858-63.Gomide,R. L. 1998. Instrumentacao e metodos de medicao lia planta. In: Gomide, R.L. edMonitoramento para manejo da irrigacao: instrumentacao, automacao e metodos. ed.: Suprema Grafica e Editora, p. 158-168.Gomide, R. L., Sediyama, G. C. 1998. Instrumentacao e metodos de medicao meteorologicos.ln Gomide, RL. ed. Monitoramento para manejo da irrigacao: instrumentacao,automacao e metodos. ed. : Suprema Grafica e Editora. P. 137-158.Gomide, R.L.; Albuquerque, P.E.P.; Kobayashi, M.K.; Inamasu, R.Y. Microprocessor andAutomation Techniques for Registration ofWater Requirements, Stress Indexes, andIrrigation Timing Criteria of Com and Beans Crops in the Brazilian South-EastIrrigated Fields. ASAF Annual lnternational Mecting, ASAE, Sacramento,California, USA. July 30-August 1,2001. Paper Numbcr: 01-20-19.Idso, S.R, Jackson, R.D, Pinter Jr., P.J., Reginato, R G. and Hatfield, J.L. 1981. Normalizingthe stress-degree-day parameter for environmental variability. Agricultura!Meteorology, Amsterdam, VoL24, pp.45-55.Inoue, Y: 1991. Remote and visualized sensing of physiological depression in crop plantswith infrared thermal imagery. Japan Agriculturat Research Quarterly, lbaraki,Vo1.25, pp.l-S.Jackson, R.D. 1982. Canopy temperature and crop water stress. Advances in irrigation. NewYork, VoU, pp.43-85.Jackson, R.D., Idso, S.B., Regi nato, R.l and Pinter Jr., P.J. 1981. Canopy temperature as acrop water stress indicator. Water Resource Research, Washington, Vo1.17, pp.1133-8.Jackson, R.D., Kustas, W.P. and Choudhury, BJ. 1988. A reexamination of the crop waterstress index. Irrigation Science, Heidelberg, Vo1.9, pp.309-17.Jensen, M.E., Burman, R.D. and Allen, R.G. 1990. Evapotranspiration and irrigation waterrequirements. New York: ASCE, 332p. (ASCE - Manual and reports on engineeringpractice, 70).Monteith.T, L. 1965. Evaporation and environment. ln: The state and movement ofwater inliving organisms New York Academic Press. Proc Symp. Soe. Exp. Bio!' 19: 205-234.Sediyarna, G c., kobayashi, M K., Gomide, R. L., Magalhães. P C, Durães, F. O M 2000Dry bean evapotranspiration rates estirnated by Penman-Monteith equation inventilated greenhouse 111:2-1thConference 011Agricultural and Forest Meteorology,Davis, Califomia, USA p.18-21.Smith, M., Segeren, A, Pereira, L.S., Perrier, A and Allen, R. 1991. Report 011 lhe experlconsultation 011 procedures for revision of FAO guidelines for prediction of cropwater requirements. Rome: FAO, 45p.

Controlling and monitoring water stress in contrasting environment for drought tolerance phenotyping of cereals and legumes.

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Controlling and monitoring water stress in contrasting environment for drought tolerance phenotyping of cereals and legumes.

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