ADER, P.

AVILA, V. S. de

KLEIN, C. H.

KRABBE, E. L.

LOPES, L. dos S.

RUIZ, J. H. de A.

English

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WPC2012 - Salvador - Bahia - Brazil • 5 - 9  August - 2012                                                                                                       Book of Abstracts  World´s Poultry Science Journal, Supplement 1, Expanded Abstract - Poster Presentation  DIETARY ENZYME SUPPLEMENTATION ON BROILER PERFORMANCE FROM 1 UP TO 42 DAYS OF AGE  Valdir Silveira de Avila1, Everton Luis Krabbe1, Leticia dos Santos Lopes1, Claudete Hara Klein1, Peter Ader2 , Juan Hilario de Araujo Ruiz3,  1Embrapa Suínos e Aves, Concórdia, SC, Brazil 2BASF SE, Lampertheim, Germany 3BASF SA, São Paulo, SP, Brazil ABSTRACT Poultry feed industry has been searching for efficient enzymes to improve feed  utilization. A performance trial was conducted to evaluate the effect of an endo-1,4-ß-arabinoxylanase and endo-1,4-ß-glucanase containing  enzyme formulation (NSPE)  in combination with various dosages of  a fungal 3- phytase (Phy) in order to show in male Cobb broiler  a nutritional value of  the enzyme combination. Broiler were housed in floor pens from day 1 up to 42 of age and submitted to four treatments: T1 = Control diet; T2 = Negative Control (reduced by 130 kcal of AMEn;  0,10% Ca and  0,13% P avail); T3 = T2 with 50 g/T of NSPE  and 500 FTU/kg Phy; T4 = T2 with  100 g/T of NSPE and  1000 FTU/kg Phy. Corn/soybean meal based  mash feed  was offered ad libitum by four phase feeding regime. After 42 days no difference was observed for feed intake (P> 0,12). T3 and T4  were not significantly different from T1 for body weight and body weight gain,  but were significantly superior to T2. Feed conversion ratio of T2  was significantly worse compared to  T1, T3 and T4 . Supplementation of the combinations of NSPE and fungal 3-phytase to broiler feed  was able  to compensate completely the nutritional deficiency  of the negative control (T2). KEYWORDS: Bird, NSP enzymes, performance, fungal 3-phytase  INTRODUCTION Approximately 90% of brazilian poultry diets are vegetable ingredients, basically corn and soybean meal. Phytic acid represents the major part of the phosphorus content present in such raw materials (Tejedor et al. 2001). Amongst others the interaction between phytic acid and proteins seems to one important reason  for the reduced digestibility  of  protein and other nutrients compared to low phytate levels containing diets (Sebastian et al. 1997).  Poultry industries have been using enzymes to improve nutrient digestibility and increase the energy value of diets. Phytase in poultry diets have provided an increase in bioavailability of calcium and phosphorus, increase in protein digestibility and increase of metabolizable energy, which is beneficial reducing the excretion of those nutrients to the environment (Avila et al.,  2010).   WPC2012 - Salvador - Bahia - Brazil • 5 - 9  August - 2012                                                                                                       Book of Abstracts  World´s Poultry Science Journal, Supplement 1, Expanded Abstract - Poster Presentation  An important part  of the carbohydrates  that reach the  intestine  of birds are  Non Starch Polysaccharides (NSPs). NSPs  are not digested due  to the presence of β-glycosidic bonds between the NSP building monosaccharides  what makes them resistant from  degradation by intrinsic digestive enzymes (TORRES, 2003). Supplementd NSP  hydrolyzing enzymes  are able to split the large NSP molecules  whereby the viscosity of the digesta is reduced  and digestibility as well as animal performance is consequently improved  (FURLAN et al., 1997). The objective of this study was to evaluate the combined effect of different dosages of a NSP enzymes formulation and a fungal 3-phytase  on broiler performance from day 1 up to 42  of age assuming a nutritional benefit for the feed formulation.   MATERIAL AND METHODS A performance trial was conducted to evaluate the effect of NSP enzymes blends (NSP = endo-1,4-ß-xylanase (EC 3.2.1.8) + endo-1,4-ß-glucanase (EC 3.2.1.4) derived from  Talaromyces emersonii)  in combination with a  fungal 3-phytase (EC 3.1.3.8) derived from Aspergillus ficuum . Day old Cobb male broiler were housed in floor pens and submitted to four treatments: T1 = Control diet; T2 = Negative Control (– 130 kcal of AMEn; - 0,10% Ca and – 0,13% P avail); T3 = T2 with 50 g/T of NSPE and 500 FTU/kg Phy; T4 = T2 with  100 g/T of NSPE and 1000 FTU/kg Phy. Diets were formulated to contain the same amount of raw material originated from vegetables. AME, Ca and P levels were attended adjusting the content of soy oil, limestone and dical-phosphate and a non-nutritive filler (Caolin). The nutritional profile is presented in Table 1. A completely randomized block arrangement was used, were block corresponded to day-old chick weight, with 10 replicates and 33 birds/replicate. Feed intake (FI), body weight (BW), body weight gain (BWG) and feed conversion ratio (FCR) were recorded.  Data were submitted to statistical analysis (ANOVA), GLM, SAS (2008). Block (day old chick weight) and treatment effect were tested, comparing means by Tukey Test, 5%.     Table 1. Nutritional profile of experimental diets.  Nutrient Pre-Starter Starter  Grower  Finisher  WPC2012 - Salvador - Bahia - Brazil • 5 - 9  August - 2012                                                                                                       Book of Abstracts  World´s Poultry Science Journal, Supplement 1, Expanded Abstract - Poster Presentation  T1 T2, T3, T4 T1 T2, T3, T4 T1 T2, T3, T4 T1 T2, T3, T4 AMEn, kcal/kg 2930 2800 2980 2850 3050 2920 3100 2970 Crude Protein, % 22.9 22.9 20.33 20.33 19.23 19.23 18.43 18.43 Calcium, % 0.72 0.62 0.67 0.57 0.61 0.51 0.51 0.41 Total Phosp., % 0.71 0.58 0.67 0.54 0.62 0.49 0.56 0.43 Avail. Phosp., % 0.45 0.32 0.42 0.29 0.38 0.25 0.32 0.19 Dig. Lys., % 1.30 1.30 1.18 1.18 1.04 1.04 0.97 0.97 Dig. Met+Cis., % 0.85 0.85 0.85 0.85 0.76 0.76 0.71 0.71 Dig. Threo., % 0.85 085 0.74 0.74 0.68 0.68 0.63 0.63  RESULTS AND DISCUSSION Performance data are presented in Table 2. After 42 days no difference was observed for feed intake (P> 0.12) between all treatments. T2 showed significantly lower performance regarding to FCR compared to T1 whereas reduction of body weight gain and body weight was numerically. T3 and T4 were significantly superior to T2 regarding to all parameters except of T4 for body weight.  The worse FCR of T2 in relation to all additional treatments was a consequence of lower body weight gain, as a response to the lower nutritional profile of the diet (T2). However, the fact that T3 and T4 did not differ statically from T1 indicates that the nutritional deficiency  of the diets were compensated by the enzyme effect. In present study the higher phytase inclusion compared to the lower one (T3 vs T4) indicates that 500 FTU/kg were already able to balance out completely the P and Ca reduction.   Table 2. Feed intake (FI) [kg/bird], body weight (BW) [kg/bird], body weight gain (BWG)[kg/bird]and feed conversion ratio (FCR)[kg/kg] of male Cobb broiler  fed from day 1  up to 42  of age.  Tratamento FI BW BWG FCR 1 4,320±0,032 2,576±0,018 AB 2,516±0,015 AB 1,770±0,008 B 2 4,368±0,040 2,533±0,033 B 2,469±0,027 B 1,828±0,009 A 3 4,432±0,048 2,620±0,029 A 2,559±0,028 A 1,789±0,008 B 4 4,403±0,039 2,610±0,029 AB 2,562±0,029 A 1,764±0,017 B Prob > F 0,1281 0,0320 0,0218 0,0001 CV, % 2,32 2,62 2,82 1,59 Means followed by distinct letter in the columns were statistically different by Tukey Test (p ≤ 0.05).  CONCLUSION The reduction of 130 kcal, 0.10% calcium and 0.13% available phosphorus in  corn-soy based broiler diet did negatively  affect broiler performance during the trial period (day 1 up to WPC2012 - Salvador - Bahia - Brazil • 5 - 9  August - 2012                                                                                                       Book of Abstracts  World´s Poultry Science Journal, Supplement 1, Expanded Abstract - Poster Presentation  42 of age). However the supplementation of 50 g/T of a NSP enzymes formulation combined with 500 FTU or 100 g/T of NSP enzymes associated with 1000 FTU, both  a fungal 3-phytase  compensated completely the nutritional suppression. Combination of the enzymes show interesting potential for optimized feed cost formulation. Further investigations are needed to evaluate more dose combinations.   REFERENCES AVILA, V. S. de; KLEIN, C. H.; BRUM, P. A. R. de; COLDEBELLA, A.; RUIZ, J. H. de A.; LIMA, G. J. M. M. de, (2010). Desempenho e qualidade da tíbia de frangos de corte criados até 42 dias de idade com dietas deficientes em fósforo e cálcio, suplementadas com fitase. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 47., 2010, Salvador. Empreendedorismo e progresso científicos na zootecnia brasileira de vanguarda: anais. Salvador: SBZ, 2010. 1 CD-ROM. FURLAN, A.C.; FRAIHA, M.; MURAKAMI, A.E.; MARTINS E.N.; SCAPINELO, C. MOREIRA, I. (1997). Utilização de complexo multienzimático em dietas de frangos de corte contendo triticale. 1. Ensaio de digestibilidade. Revista Brasileira de Zootecnia; Viçosa, v. 26, n4, P. 765-772. SAS Institute Inc. (2008). SAS/STAT® 9.2 User’s Guide. Cary, NC: SAS Institute Inc. SEBASTIAN, S.; TOUCHBURN, S. P.; CHAVEZ, E. R.; LAGUE, P. C. (1997). Apparent digestibility of protein anda mino acids in broiler chickens fed a corn-soybean diet supplemented with microbial phytase. Poultry Science, v. 76, n. 12, p. 1760-1769. TEJEDOR, A. A.; ALBINO, L. F. T.; ROSTAGNO, H. S.; LIMA, C. A. R.; VIEITES, F. M. (2001). Efeito da adição de enzimas em dietas de frangos de corte à base de milho e farelo de soja sobre a digestibilidade ileal de nutrientes. Revista Brasileira de Zootecnia, v. 30, n. 3, p. 809 - 816. TORRES, D. M. (2003). Valor nutricional de farelos de arroz suplementados com fitase, determinado por diferentes metodologias com aves. 172p. Tese (Doutorado em Zootecnia) – Universidade Federal de Lavras, Lavras, MG. 

Dietary enzyme supplementation on broiler performance from 1 up to 42 days of age.

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Dietary enzyme supplementation on broiler performance from 1 up to 42 days of age.

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