CARRA, M. L.

DAL’BÓ, G. A.

GONÇALVES, V. T.

LOSEKANN, M. E.

LUIZ, A. J. B.

QUEIROZ, J. F. de

SAMPAIO, F. G.

WOLF, V. H. G.

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5° Simpósio Internacionalde Nutrição e Saúde de PeixesFMZ - UNESP - Botucatu. 6 a li de novembro de 2013Hematology and blood morphometric partern of four tropical fish species with economicalimportanceGenoefa Amália DaJ'Bó*l, Femanda Garcia Sampaiol, Verena Hildegard Gyárfas Wolf2, JulioFerraz de Queirozl, Marcos Eliseu Losekannl, ,Alfredo José Barreto Luizl, Vitória TeodoroGonçalves3, Maria Lidia Carra3* Pós Graduanda em Patologia Clínica Veterinária; Instituto Brasileiro de Veterinária; bolsista; lPesquisadores,Empresa Brasileira de Pesquisa Agropecuária -Embrapa Meio Ambiente; Rod SP 340, km 127,5 -13820-000-Jaguariúna - SP - fernanda.sampaio@embrapa.br; 2Pesquisador, Laboratório de Medicina Preventiva, Faculdade deJaguariúna, Jaguariúna - SP; 3Graduandas em Engenharia Ambiental, Faculdade de Jaguariúna, Jaguariúna - SP.Aquaculture has become one of the most important activities of Brazilian agribusiness. The useand validation of standardized non-lethal and expressive methods to monitor the status of fishhealth is being considered a major issue to assure the expansion of fish production. Thus,establishment of hematological pattems should be used as a reference to evaluate the status offish health to be considered one of the main tools to assess the status of health index of differentspecies. The basal hematological profile ofjundiá (Rhamdia quelen), Nile tilapia, piracanjuba(Brycon orbignyanus) and pacu (Piaractus mesopotamicus) has been characterized. Fishes weremaintained during 30 days in experimental glass aquariums, in closed circulation system, wellaerated, with constant temperature. The water quality parameters in the aquarium were dailymeasured. At the end ofthe experimental period, fish were anesthetized and blood was collectedfrom caudal puncture with heparinized syringe, for hematological analysis. The hematologicalprocedures of erythrogram and leukogram, and the measured of larger (LA) and minor axes(MA), surface (SF) and volume (VL) ofred blood cell (RBC) were done. Data are presented asmean and coefficient ofvariation (CV %), the minimum and maximum values, and theoccurrence (%). No mortality was observed in any experimental group. In the present study thepiracanjuba presented the highest means of hematocrit (Ht), RBC and mean corpuscular volume(MCV) in comparison to the other species. Piracanjuba also presented the higher value ofHt,hemoglobin (Hb) and MCV. However, Nile tilapia presented the minor mean and value ofHt,Hb, RBC and mean corpuscular volume (MCHC) when compared to the other three species. TheHt and RBC range data of piracanjuba do not match values from the other three species analyzedin the present study. The minimum and maximum range ofRBC, Ht and Hb ofNile tilapia,jundiá and pacu are included in the same interval. The ranges of MCV and MCHC for the fourspecies were in the same intermission period. Jundiá presented the highest CV ofHt, Hb andRBC, with higher amplitude of data ofHt and Hb. Pacu presented the lesser amplitude of datafor Ht, Hb and MCV. In the present study ali the fish species has showed WBC in a similarrange. The jundiá and Nile tilapia were the species with the higher variety for white blood cell(WBC), neutrophils (N0), Iymphocyte (L0), monocyte (M0) and trombocyte (Trb). In thepresent study the L0 was the most common leukocyte observed in the studied species, followedby N0, M0 and eosinophils (E0). The jundiá was the unique specie that presented basophils(B0). The Trb was the most common cell found in the studied species after RBC. The jundiápresented higher mean and value ofTrb. Data ofLA, MA, SF and VL from studied species in thepresent study are in the same range of variation. Some mistakes and lack of standards foranalytical methods cause the comparison of resuIts between authors and species very difficult.Despite some differences the pacu presented the higher values of LA, MA, SF and VL whencompared to the other species studied. The erythrocyte is a common cell, but the cell size canvary in function of the large number of fish species. RBC size can varies inversely with wholeanimal metabolic activity. It was possible to observe that the piracanjuba presented higherhemoglobin concentration, and that it is not the species with larger RBC.1

Hematology and blood morphometric pattern of four tropical fish species with economical importance.

http://www.alice.cnptia.embrapa.br/alice/bitstream/doc/982563/1/2013RA055.pdf

Hematology and blood morphometric partern of four tropical fish species with economical importance.

Hematology and blood morphometric partern of four tropical fish species with economical importance.

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