FRIZZO, C.

QUOIRIN, M.

SANTOS, I. R. I.

VIEIRA, E. S. N.

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In vitro germination of embryonic axes of Araucaria angustifolia (Bertol.) Kuntze var. angustifolia and var. caiova.

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In vitro germination of embryonic axes of Araucaria angustifolia (Bertol.) Kuntze var. angustifolia and var. caiova FRIZZO, C 1 ; SANTOS, I.R.I.2; VIEIRA, E. S. N.3 ; QUOIRIN, M.4  1Aluna de Mestrado do Programa de Pós-Graduação em Agronomia – Produção Vegetal – Universidade Federal do Paraná, Campus Agrárias – Rua dos Funcionários, 1540 – Juvevê – CEP 80035-050, Curitiba, PR, Brasil. eng.carolinefrizzo@gmail.com 2 Pesquisadora Embrapa Recursos Genéticos e Biotecnologia – Embrapa Recursos Genéticos e Biotecnologia. Avenida W5 Norte (Final) Caixa Postal 02372 Brasília – Distrito Federal CEP: 70770-917 izulme.santos@embrapa.br 3Pesquisadora Embrapa Florestas – Estrada da Ribeira, km 111 CEP 83411-000, Colombo, PR, Brasil. elisa@cnpf.embrapa.br 4Doutora, professora, Departamento de Botânica, Setor de Ciências Biológicas – UFPR, Av. Coronel Francisco H. dos Santos, s/n., Caixa Postal , 81531-980, Curitiba, PR. mquoirin@ufpr.br  Araucaria angustifolia (Bertol.) Kuntze, Brazilian pine, is a native tree species of major economical and ecological importance, particularly in the southern region of the country. It is extensively logged due to its high quality wood and harvested for its edible seeds similar to large pine nuts. The main objective of this study was the establishment of a methodology for in vitro germination of embryonic axis of two varieties: angustifólia and caiova. The seeds used in the experiments were harvested at Embrapa Forestry in Colombo-PR and stored in a chamber at 10°C and 95% relative humidity for a period of four months (angustifólia) or one month (caiova). Subsequently they were taken to the Plant Cryobiology Laboratory at Embrapa Genetic Resources and Biotechnology at Brasília-DF to carry out the current study. Firstly, the external seed tegument was removed and the seeds were disinfested using a commercial solution of sodium hypochlorite (2% active chlorine) for 15 minutes and rinsed three times with autoclaved distilled water.. Afterwards the embryos were excised from the seeds, the cotyledons were removed and the embryonic axes were transferred to culture tubes containing WPM culture medium supplemented with 3% activated charcoal. The axes were then cultured in a growth room at 25±2º C a 16 h photoperiod. At the second day of growth, i  the onset of the embryonic axis elongation was observed; at the fourth day the beginning of greening and at the seventh day the axes were well grown. The germination percentage was 80% for A. angustifolia var. caiova and 70% for var. angustifolia. The methodology used for the in vitro germination of axis of these two varieties proved to be efficient. As a follow up to this work some studies are now carried over in order to develop a cryopreservation methodology for the embryonic axis of both varieties.   

In vitro germination of embryonic axes of Araucaria angustifolia (Bertol.) Kuntze var. angustifolia and var. caiova.

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Repository Open Access to Scientific Information from Embrapa