BING-CHI HUYNH, J.

FARINAS, C. S.

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IJ1r••\ ia \ J ruod« ( (/I. t ca I mhr Ipf \(11. Cari-». - O~ I' (19de (I '(I,to de )fJl ~I { Ibl aJ 1'1cuan« 'lItl'"/'. e Fmbr ipa Insttumc ntacã» - "ao ( arte I - "P - BrasilEvaluating solid-state fermentation for cellulases production usingTrichoderma harzianumJackie Bing-Chi Huynhl; Cristiane Sanchez Farinas2I Jackie Bing-Chi Huynh Master's of Science in Engineering, Engineering Education, Purdue University, WestLafayette, Indiana, United States jhuynhtêpurdue.edu.2 Embrapa Instrumentation, Rua XV de Novembro 1452, CEP 13560-970 São Carlos, São Paulo, BraziLTrichoderma harzianum was cultivated using different sets of conditions for solid-statefermentation.. These experiments were carried out to understand the behavior of Trichodermaharzianumfor cellulases production. (FPase, Xylanase, endoglucanase (CMCase), ~-glucosidade, and protein). Solid-state fermentations are known to produce more activecellulases at a cheaper cost compared to submerged ferrnentations. Preliminary kineticexperiments were carried out in flask cultures using 5 gram of wheat bran, Mandei nutrientmedium at a humidity of 66%, and inoculated with 107 spores to be grown at 29°C.Afterward two further kinetic studies were carried out using flask cultures, which werewashed wheat bran and different humidities (30%, 40%, 50%, and 60%). An initial dataanalysis resulted in a 2 fold increase from preliminary data. These results are competitive tobenchmark values compared with submerged fermentations. More experiments needs to becarried out using different solid medium, temperature, and liquid medium to truly obtain theoptimal conditions for Trichoderma harzianum. Solid-state fermentations hold an enormouspotential to decrease the cost of cellulases production, thus allowing further development ofsecond-generation biofuel. Currently, bioreactors are being developed for solid-statefermentation for large scale production. Combining genetically engineered Trichodermaharzianum, optimal growth conditions, and large scale production will have an advantage rolein cellulose biofuel.Apoio financeiro: Embrapa.Área: Agroenergia.5

Evaluating solid-state fermentation for cellulases production using trichoderma harzianum

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