FERRARIS, F. K.

HENRRIQUES, M. das G. M. O.

MELO, I. S. de

SANTOS, S. N.

SOUZA, A. O. de

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  Endophytic fungi from Combretum Ieprosum with potential anticancer and  antifungal activities  Suikinai Nobre Santos+", Faustos Klabund Perraris", Ana Olivia de Souza", Maria  das Graças M. O. Henriques", Itamar Soares Melo1,2  I Laboratório de Microbiologia Ambiental- Embrapa Meio Ambiente (CNPMA), Via SP 340,  Km 127,5, c» 69, CEP: 13820-000, Jaguariúna, st. Brasil; e-mail: itamar@cnpma.embrapa.br  2 Departamento de Ciência do Solo - Escola Superior de Agricultura 'Luiz de Queiroz', Univer-  sidade de São Paulo (USP), Avenida Pádua Dias, 11, CP. 83, CEP: 13400-97, Piracicaba, Sp,   Brasil  '  3 Laboratório de Química de Produtos Naturais Farmanguinhos, Fiocruz Rio de Janeiro,  Brasil  4 Laboratório de Bioquímica e Biofísica, Instituto Butantan, Av. Vital Brasil, 1500, São  Paulo, Sp, Brasil  There is a general call for new antibiotics, chemotherapeutic agents and agrochemicals  that are highly effective and possess low toxicity, Large number of microbial sources  have been tested for production of bioactive compounds. Among these microorgan-  isms, some endophytic fungi have been studied as rich source of bioactive compounds.  The present study deals with the isolation of endophytic fungi from Combretum  leprosum (Combretaceae family), and the evaluation of the potential anticancer and  antifungal activities. This plant is used in folk medicine to treat haemorrhages, and  as a sedative. The fungal isolates were identified by sequencing the 18S rDNA region  and their dichloromethane extracts were investigated for in vitro anticancer activity,  determining the growth inhibition and cytotoxic properties against seven cancer cell  lines (HeLa, ECV304, B16F10, J744, P388, Jurkat and k562). The antifungal activity  were tested against Candida albicans, Candida krusei, Candidaglabrata, Candidaguiller-  mondi, Candida tropicalis, Cryptococcus neoformans and Trichophyton rubrum. In the in  vitro antitumor assay, the crude extract of a fungal strain, identified as Aspergillus sp.  CFE103, showed significant cytotoxicity effects against the cancer lines J744 with ICso  of 0.80 and leukemia (Jurkat) with ICso of 0.89. Among the solid tumors, the highest  inhibition occurred against ECV304 with ICso of 3.08 and (HeLa) with ICso of 2.97.  The extract of another endophytic strain, identified as Fusarium oxysporum, showed  high levels of inhibition against P388 with ICso of 2.14 and J744 with ICso of 2,98. The  minimum inhibitory concentrations were observed between 4-125 ug/rnl.", being the  most active compounds obtained from F. oxysporum CFE177 against C. glabrata, C.  neoformans and T. rubrum. It is concluded that compounds that showed better anti-  fungal activities were those that showed the most active antitumor activity, and that  Combretum leprosum, hosts promising source of endophytes with potencial anticancer  and antifungal activities.   July 22-28, Kraków, Poland   317     Melo, Itamar Soares  Mendel, Zvi  Meng, Xian-Ying  Menu, Frédéric  Mergaert, Peter  Merlos, Miguel Angel  Merrild, Marie  Merville, Adrien  Mesjasz- Przybylowicz, Jolanta  Metcalf, Jason A.  Metrak, Monika  Miadlikowska, Jolanta  Miao, Vivian aw  Michalik, Anna  Miché, Lucie  Michelsen, Anders  Minaeva, Katherina  Mironov, Timofey  Mishra, Raj K.  Miszalski, Zbigniew  Mitchell, B. Greg  Mleczko, Piotr  Moczek, Armin  Mogildea, Daniela  Molan, Peter  Monk, Jana  Moora, Mari  Morag, Neta  Moran, Nancy A.  Moreau, Pierre-Arthur  Moretti, Beatrice  Morin, Emmanuelle  Morin-Adeline, Victoria  Moriyama, Minoru  Mosandl, Reinhard  Mosquera, Ana Teresa  Mostafavi, Pargol Ghavam  Moszczyríski, Krzysztof  Motta, Maria Cristina M.  Mouton, Laurence  Moya, Andrés  Mozes-Daube, Netta  358   22,317,334  21,289  26,109  24,82  12,96,334  18,250  29,218,334  24,82,334  29,34,222,262,334  13,122,335  24,84  18,226,227,335  24,80  27,61  30,237  29,218  26,59  26,114  14,147  34,264  17,205  3,29,219,335  18,232  29,220,335  20,284  20,287  32,247  19,270  13,119  11, 70  14,151  13,129  21,314  19,266  21,308  29,174  29,221,327  24,125  26,110,335  13,131  13,24,81,87,131  20,274  7th International Symbiosis Society Congress   

Endophytic fungi from Combretum leprosum with potential anticancer and antifungal activities.

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