Isolation and identification of caprine lentivirus in synovial fluid and blood using Nested PCR and western blotting.

Abstract

The caprine arthritis-encephalitis (CAE) is an infectious, multisystem disease, caused by a retrovirus belongs to the Lentivirus genus, which affects all ages goats and breed types. This study aimed to investigatethe caprine arthritis-encephalitis virus (CAEV)through the isolation of native strains and identification with polymerase chain reaction (nested PCR) and western bloting (WB)