ABREU, D. A.

ANDRIOLI, A.

BRITO, I. F.

BRITO, R. L. L.

DIAS, R. P.

PINHEIRO, R. R.

SIDER, L. H.

SOUZA, K. C.

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Clinical and Serological Monitoring of Goats Experimentally Infected by Virus Arthritis-Encephalitis CaprineSouza, K.C.1, Andrioli, A.2, Pinheiro, R.R.2, Brito, R.L.L.2, Dias R.P.2, Brito, I.F.1, Abreu, D.A.1, Sider,L.H.21. UVA, Universidade Estadual Vale do Acaraú/Embrapa.   2. CNPC, Embrapa Caprinos e Ovinos.kelma_zoo@hotmail.comAbstract / Resumo:The objective of this study was to evaluate which diagnostic test, agar gel immunodiffusion (AGID) orWestern Blot (WB), would detect early seroconversion of goats experimentally infected by Caprine Arthritis-Encephalitis Virus (CAEV) via artificial insemination and relate seroconversion with clinical signs of diseaseThirty goats were used, with no defined breed, inseminated with semen contaminated with the standard virusstrain CAEV-Cork, with infective titles of 106TCID50/mL and another 102TCID50/mL. The goats were thenserologically and clinically followed for a year. The experiment was conducted in accordance to the ethicalprinciples of animal experimentation. Statistical analysis was performed using the chi-square test (P <0.05).At 30 days after insemination, there were found the first seroconvertions. In two, out of 20 goats inoculated,antibodies were detected for the CAE virus using the technique of AGID, and 12 by a WB. After 60 days ofthe AIs, all inoculated goats showed reaction of antibodies to antigens of CAEV in WB and in one year inboth tests, indicating that they were infected. All the 10 goats in the control group remained seronegativethroughout the experiment. The AGID presented, during the study period, eight false-negative results among60 tests (13.3%), while the WB showed only one false-negative among 132 tests performed (0.76%) (P<0.001). As the clinical symptoms of CAE noted that only two (10%) of the infected females showed a slightincrease in the articular index clinical, are considered clinically suspected joint problems It was concludedthat due to CAE remains asymptomatic for long periods, and the AGID test does not detect early infectedanimals, and the high occurrence of false-negative, there should be used more sensitive diagnostic tests, suchas WB, periodically, to monitor the CAE in the national herd.

Clinical and serological monitoring of goats experimentally infected by Virus Arthritis-Encephalitis Caprine.

Clinical and serological monitoring of goats experimentally infected by Virus Arthritis-Encephalitis Caprine.

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