BOTELHO, C.

FARIA-CAMPOS, A. C.

FERNANDES-RAUSCH, H.

FOLGUERAS-FLATSCHAR, A. V.

NODA, R. W.

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Topic: Databases and Bioinformatics ToolsPI: DBT04DEVELOPING A WORKFLOW FOR MANAGEMENT OF INFORMATIONFROM A PLANT TRANSFORMATION FACILITYBotelho c', Folgueras-Flatschart A v: Faria-Campos A C1, Fernandes-Rausch H1, Noda R W2,Carneiro A A 2, Campos S V A 1+Loboraiárío de Universalização de Acesso - DCC - UFMG2Núcleo de Biologia Aplicada - EMBRAPA Milho e SorgoGood Laboratory Practices, or GLP, are a set of management principIes of research laboratoriesactivities that provide a framework within which studies are planned and performed in order to ensureresults consistency and reliability. ln order to enforce the GLP adoption, it is important to demonstratethat the information produced and processes applied to obtain it are correct and traceable. Therefore,the use of automated systems to folJow each step of the research in a laboratorycan be of significantassistance. These systems are called Laboratory Information Management Systems (LIMS) and are nowoften used in research laboratories. In this work we describe the use of a LIMS based on workflowsto manage the data from the Plant Genetic Transformation Laboratory at EMBRAPA Milho e Sorgo,that works on the analysis and production of transgenic maize and sorghum plants. Transgenics areorganisms whose DNA is modified using genetic engineering aiming to introduce a new characteristicwhich does not occur naturalJy in that species. We have developed a workflow to manage the informationfrom the processes to obtain genetically modified plants to be used in the LIMS Flux (Satya Sistemas).A workflow in XPDL format was constructed using the Together Workflow Editor tool and imported intothe system where data are stored in a MySQL database. This workflow models the data and the activitiesof maize transformation mediated by Agrobactenum tumefaciens, in which the user defines the geneticconstruction specifications, the plant genotypes and conditions for the transformation, transgenic callusselection and plant regeneration. Finally, from workbench to glasshouses, the system stores experimentalprotocols and data from the whole processes up to obtaining and storing modified seeds, thus ensuringthat GLP are followed. This work presents a solution for the management of information produced bya laboratory working with transgenic plants and our hope is to contribute with a quality improvement ofthe produced data. Supported by: FAPEMIG, CNPq.106

Developing a workflow for management of information from a plant transformation facility.

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