DOMINGUES, I.

JONSSON, C. M.

MOURA, A. M.

NOGUEIRA, A. J. A.

OLIVEIRA, R.

Repository Open Access to Scientific Information from Embrapa

59ASSESSING MIXTURE TOXICITY OF HERBICIDES USING ZEBRAFISH (DANIORERIO) EARL Y-LIFE STAGESM.A.M. Moural,2, L Domingues/, R. Oliveira", C.M. Jonssorr', A.lA. Nogueira"IInstituto Biológico, Lab. Ciência das Plantas Daninhas, Campinas, SP, Brasil;2CESAM e Departamento de Biologia, Universidade de Aveiro, Campus Universitário deSantiago, 3810- 193 Aveiro, Portugal3Embrapa Meio Ambiente, Jaguariúna, SP, Brasile-mail: monica_moura@biologico.sp.gov.brHerbicides are the most common pesticide used in agriculture, but the impact of this practiceon non-target organisms isn't well known. In the environment these compounds are rarelyalone, but almost no information is available about the combined toxicity of herbicides. Theaim of this work was to evaluate the effects of the mixture of ametryn and tebuthiuron, twoherbicides widely used in sugarcane cultivation, the predominant crop of the state of SãoPaulo, Brazil. The toxicity of the mixture was evaluated using the Fish eleutheroembryotoxicity (FEET) test, with Danio rerio, a tropical cyprinid used in ecotoxicological research.The endpoints monitored incIuded pigmentation, otolith formation, yolk absorption,pericardial oedema, tail deformation, hatching, coagulation and death. Five concentrations ofametryn (ranging from 19.44 to 72.91 mg L-I) and tebuthiuron (ranging from 0.13 to 0.47 g L-I) were tested. Statistical analysis was performed with ToxCalc spreadsheet built overMicrosoft Excel. Independent Action and Concentration Addition models were used to fit thedata. Binary mixture data was used to identify possible deviations from each model and thebest fit was selected based on statistical comparisons between outputs. Ametryn is most toxicthan tebuthiuron, presenting a LCsO-96h of 52.94 (± 5.70) mg L-I and 413 (± 60) mg L-I,respectively. Ametryn and tebuthiuron show antagonism when used as binary mixture and themodel that best describes this mixture is the Independent Action. The endpoints evaluated onthis work were useful to understand the mode of action ofthe binary mixture and reinforce theimportance of monitoring sublethal parameters to assess the real risk of agrochemicals foundon aquatic ecosystems.Keywords: Fish eleutheroembryo toxicity test, mixture toxicity, ametryn and tebuthiuronApoio: FAPESP (Bolsa Novas Fronteiras Proc. 2010/07118-9) e CESAMlDepartamento deBiologia, Universidade de Aveiro, Portugal.10 a 12 de outubro de 2011UNESP - Instituto de 8iociênciasRio Claro / SP

Assessing mixture toxicity of herbicides using zebrafish (Danio rerio) early-life stages.

http://www.alice.cnptia.embrapa.br/alice/bitstream/doc/919758/1/2011RA107.pdf

Assessing mixture toxicity of herbicides using zebrafish (Danio rerio) early-life stages.

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