CASTRO, J. M. da C. e

FERNANDES, K. V. S.

LIMA FILHO, J. L.

OLIVEIRA, E. A. G.

PINTO, M. S. T.

RIBEIRO, J. M.

In this context, the objective of the present study was to look for genes related to resistance to M. mayaguensis in peanut plant

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Prospection of Resistance Genes to Meloidogyne mayaguensis in Peanut Plants (Arachis hypogaea)Ribeiro, J. M.1; Castro, J. M. C.1; Melo, N. F.1; Lima Filho, J. L.2; Oliveira, E. A. G.1; Pinto, M. S. T.3; Fernandes, K. V. S.  3 1Embrapa Semi-Árido, Petrolina-PE; 2Universidade Federal de Pernambuco, Recife-PE; 3Universidade Estadual do Norte Fluminense, Campos dos Goytacazes-RJ; Brazil.In several areas of Brazil, including the Sao Francisco River Valley, Meloidogyne mayaguensis has been the main responsible for guava trees death, resulting in significant losses in the production of this fruit crop. Up to now, chemical products experimentally  tested  have  not  been  efficient  and  there  are  no  nematicides registered in the Ministry of  Agriculture,  Livestock and Food Supply for  use in guava.  Based  on  the  difficulties,  search  for  resistance  factors  against  this nematode in immune plants,  like peanuts,  would be of great importance to the biotechnology  area,  seeking  for  immunity  in  susceptible  plants.  Among  plant defense mechanisms against pathogen infection, those mediated by R genes are the best characterized so far. Most of the proteins expressed by these genes have a  central  domain  composed of  a  nucleotide  binding  site  (NBS),  followed  by a terminal leucine rich region (LRR) on the C-terminal region. In this context,  the objective of the present study was to look for genes related to resistance to  M. mayaguensis in  peanut  plants.  For  this  purpose,  peanut  genomic  DNA  was extracted and a PCR reaction was performed using specific primers for the NBS (Nucleotide Binding Site) region of the R genes. A positive amplification of a 500 bp DNA fragment was achieved by using such strategy. The fragment was then automatically sequenced and the resulting data was compared with other R genes sequences available within the GenBank database. Key words: R genes, root-knot nematodes, Meloidogyne mayaguensis, peanut.

Prospection of resistance genes to Meloidogyne mayaguensis in peanut plants (Arachis hypogaea).

http://www.alice.cnptia.embrapa.br/alice/bitstream/doc/257442/1/OPB2446.pdf

Prospection of resistance genes to Meloidogyne mayaguensis in peanut plants (Arachis hypogaea).

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