CRUZ, N. A.

FALLEIROS, A. M. F.

FORGHIERI, L. R.

GALLO, A. R. L.

HOFFMANN-CAMPO, C. B.

LEVY, S. M.

MOSCARDI, F.

SILVA, L. X.

SILVA, M. R.

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P - 39Effects of the Flavonoids Rutin and Genistin in the Midgutof Anticarsia gemmatalis (Hübner, 1818) (Lepidoptera:Noctuidae) Larvae:Histochemical StudyLevy, S.M.l, Cruz, N.A.2, Forghieri, L.R.l, Silva, M.R.l, Gallo, A.R.Ll, Silva, L.Xl,Hoffmann-Campo, CW, Moscardi, P, Falleiros, A.MJlE-mail: sheilalevy@uel.brIUniversidade Estadual de Londrina CCB, Departamento de Histologia- Paraná/Brasil2 Universidade Estadual de Londrina CCA Departamento de Agronomia3 CNPSoja EMBRAPA Londrinaln this study we verified the chemical components of the epithelial cells of the midgut ofAnticarsia gemmatalis larvae after the ingestion of soybean genotypes resistant to insects.The caterpillars, obtained at Embrapa/Soja (Londrina-PR-Brazil), were fed withgenotypes that present different concentrations of rutin and genistin: BRS 257 (control),BR16 (0,0016mg/g genistin), Dowling (0.0429 mg/g genistin), Pl229358 (0.0212-0.0136mg/g de rutin/genistin), IAClOO (0.0972-0.0142 mg/g de rutin/genistin) e Pl227687(0,3682-0,0122mg/g de rutin/genistin). The midguts were collected, fixed and pro-cessed for histochemical analyses to detection of acids and neutral polysaccha-rides, proteins and lipids. The neutral polysaccharides were visualized specially incolumnar cells, being the greatest staining observed in insects fed with the controlgenotype (BRS257). After diastase, it was detected glycogen in epithelial cells of larvaefed with IAClOO.For total proteins technique, we observed positive labeling in columnar,goblet and regenerative cells for almost all genotypes, being the goblet cells the most reac-tive celIular type in insects treated with Dowling, similar to results detected in the controlgenotype. No labeling was visualized for acid carbohydrates and lipids. We concludethat the columnar cells of midgut in A. gemmatalis larvae presents neutral car-bohydrates, especially those structural, while the proteins were detected in gobletcells. Besides, the different concentration of the phenolic compounds rutin andgenistin in the resistant genotypes were responsible by the changes in chemicalcomposition of midgut epithelial cells, which could affect the digestive processo133

Effects of the flavonoids rutin and genistin in the midgut of anticarsia gemmatalis (Hubner, 1818) (Lepidoptera: noctuidae) larvae: histochemicla study.

http://www.alice.cnptia.embrapa.br/alice/bitstream/doc/869918/1/HC1330001.pdf

Effects of the flavonoids rutin and genistin in the midgut of anticarsia gemmatalis (Hubner, 1818) (Lepidoptera: noctuidae) larvae: histochemicla study.

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