ARAGÃO, F. J. L.

FARIA, J. C.

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 6th International Geminivirus Symposium  Poster Session                               Geminiviridae Management / Control Interactions; CNPq, FAP-DF Virus Family: Geminiviridae  Category: Management and Control  Title: The biosafety of siRNA transgenic common beans resistant to Bean golden mosaic virus.  Authors: J. C. FARIA1, F. J. L. ARAGÃO2  Address: 1Embrapa Arroz e Feijão, Caixa Postal 179, Santo Antônio de Goiás, GO CEP 75375-000 - Brasil; 2Embrapa Recursos Genéticos e Biotecnologia, Caixa Postal ----, Brasília, DF CEP     - Brasil.   Abstract: Bean golden mosaic virus (BGMV) is an important Begomovirus distributed in most of the bean growing areas in Brazil. Disease losses can be up to 100% in a singly early affected field. We recently showed that transgenic beans expressing a small interfering RNA derived from the rep gene successfully prevents plants from infection by BGMV both under high inoculation pressure in the greenhouse or in the field. According to the Brazilian law regulating on the biosafety of Genetically Modified Organisms the biosafety of GMOs shall be based on the Molecular Characterization (including protein biosafety), Agronomic and Environmental evaluation, and Substantial Equivalence. The transgene is present as two copies in a single locus, and was inherited as a single gene in a Mendelian way. Up to 30% of heterozygous plants may be infected by BGMV thus showing gene dosage effect. However, homozygous plants are completely resistant. Except for resistance to BGMV the transgenic line Olathe 5.1 was essentially like its counterpart Olathe Pinto in all required analysis. The complete set of data will be submitted to The National Technical Biosafety Commission (CTNBio) by November 2010 for analysis and a possible authorization for commercial release.   Financial support: Embrapa/Monsanto   

The biosafety of siRNA transgenic common beans resistant to Bean golden mosaic virus.

RCAAP - Repositório Científico de Acesso Aberto de Portugal

http://www.alice.cnptia.embrapa.br/bitstream/doc/875076/1/jcf1.pdf

The biosafety of siRNA transgenic common beans resistant to Bean golden mosaic virus.

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