Resumen del póster presentado al IX Meeting of the Spanish Society for Developmental Biology celebrado en Granada del 12 al 14 de noviembre de 2012.Peer Reviewe

Ariza-Cosano, Ana

Bessa, José

Gómez-Skarmeta, José Luis

Digital.CSIC

Sox21 is required for the posterior lateral line patterning by regulating FGF signalling abstract ID: 67 Main author       Author: Ana Ariza Cosano  Affiliations       1.- Andalusian Center for Developmental Biology, CSIC, Pablo de Olavide University Authors       (1) Ariza-Cosano, Ana        (1) Gómez-Skarmeta, José Luis       (1) Bessa , José   The posterior lateral line (PLL) is a mecanosensory organ present in fishes and amphibians. During development, this structure is formed from a group of cells that migrate from a region near  the otic vesicle to the tip of the tail, leaving neuromasts in its trail. Patterning in the PLL primordium occurs mainly by the antagonistic activity of two morphogens, Wnt and FGF.Two morphogens play a crucial role in PLL development. Wnt is requiered to maintain the proliferative state of cells and the counteracting FGF triggers the first steps of differentiation and epithelialisation(1). Currently upstream regulators of these two pathways remain unknown. Several genes of the Sox family are also expressed in the PLL primordium, among them sox21a, a gene required to the transition of progenitors to differentiated neurons in the vertebrate central nervous system (2), although its function in the PLL development is not yet known.  Recently it has been shown that this gene in central nervous system trigger proliferated cells into differentiated state.Recently, we started to study the role that sox21a may play during PLL development. Here we present a functional assay of sox21a in the zebrafish PLL development. To that aim we performed knockdown experiments using morpholinos and in vivo reporters as readouts. In these assays we observed a delay in PLL migration and impairment of epitheliarization. In addition, in this same sox21a morphant condition, we observed a down regulation of specific molecular markers downstream of FGF, suggesting that sox21a is an upstream regulator of this signalling. These phenotypes are similar to loss of function of FGF.  To that end, we postulate that sox21a is an upstream regulator of FGF signal in PLL. In total, we identify sox21a as new important player in the patterning of the PLL by controlling FGF signalling.  (1)    Andy Aman and Tatjana Piotrowski (2008). Wnt/-β-Catenin and Fgf signaling control collective cell migration by restricting chemokine receptor expression. Developmental Cell., 15, 749–761 (2)    Magnus Sanderg, Magdalena Källstrom and Jonas Muhr (2005). Sox21 promotes the progression of vertebrate neurogenesis. Nature Neuroscience., 8,995-1001  Acknowledgements: We are grateful to Miguel Allende for a stimulating discussion and critical comments on this work and to Fernando Casares for providing antibodies. Finally, we thank Maria Nicolas and Katarina Garcia for technical assistance with the in vivo assays.        122 ·BACK TO INDEX

Sox21a is requiered for posterior lateral line patterning by regulating Fgf signalling

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